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Hydrolyzed Dextran (5) (KS-802)

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The change of hyrolyzed dextran is analyzed. The analysis was done using SUGAR KS-802.

(Sample preparation)
1) Hydrolyze 4% dextran aquaous solution using 1M HCl at 100°C.
2) Take sample before and after 30, 40 and 50min from the start of the hydrolyzation.
3) Neutralize the sample adding cold 1M NaOH aquaous solution.
4) Desalt the sample passing it through ion-exchange resin.
5) Filtrate with 0.45μm membrane filter.


Sample : Hydrolyzed dextran, 20μL

 

Columns      : Shodex SUGAR KS-802(8.0mmI.D. x 300mm) x 2
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80°C