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Analysis of Trisaccharides (1) (NH2P-40 3E)

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The trisaccharides melezitose, raffinose, and maltotriose were separated using Asahipak NH2P-40 3E, a polymer-based amino column.


Sample : 0.1% each, 10μL
1. Melezitose
2. Raffinose
3. Maltotriose


Column       : Shodex Asahipak NH2P-40 3E (3.0mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 0.35mL/min
Detector     : Shodex RI
Column temp. : 30℃