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Cyclodextrin in Plasma (C8P-50 4E)

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This is an example of a sensitive detection of cyclodextrin contained in plasma by pulse-amperometric electrochemical detector (PAD) using strong alkaline reagent mixed with eluent at post-column. Asahipak C8P-50 4E (a column for reversed phase chromatography) is used for the separation.


Sample : β-Cyclodextrin
1. G-β-CD
2. β-CD

Columns      : Shodex Asahipak C8P-50 4E (4.6mmI.D. x 250mm)
Eluent       : 1mM Sodium hydroxide + 0.6% CH3CN
Reagent      : 1.5M Sodium hydroxide
Flow rate    : (Eluent); 0.6mL/min, (Reagent); 1.0mL/min 
Detector     : PAD
Column temp. : 50°C
Y.Kubota et al., Analytical Biochem., 201 (1992) 99.