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LC/MS Analysis of Streptomycins (VC-50 2D)

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Streptomycin which is an aminoglycoside antibiotic, and its reduced form dihydrostreptomycin were analyzed using HILICpak VC-50 2D, a polymer-based HILIC mode column, with LC/MS detection. Generally, ODS columns are used for this type of analysis, but it takes time to stabilize the column due to its requirement of an ion pair reagent. The analysis using VC-50 2D does not require an ion pair reagent, allowing the antibiotics to be separated under cation exchange mode.


Sample : 20μL
50ng/mL each (in H2O)
Streptomycin sulfate
Dihydrostreptomycin sulfate


Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : 0.25M HCOOH aq./CH3CN=90/10
Flow rate    : 0.3mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 40°C