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Food Containing Vitamins (DE-413)

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Vitamins in food were analyzed using RSpak DE-413, a column for reversed phase chromatography.

(Sample pretreatment)
- Dissolve 80mg of the food containing vitamins in 12mL of the eluant (A).
- Pass through a 0.45μm filter before injecting into HPLC.


Sample: 20µL


Quantitative resultsConcentrationContents in 4g
1. Vitamin C
145.8μg/mL
87.5mg
2. Vitamin B6
21.2μg/mL
12.7mg
3. Vitamin B2
7.3μg/mL
4.4mg
Column       : Shodex RSpak DE-413 (4.6mmI.D. x 150mm)
Eluent       : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
               (B); H2O/CH3CN=50/50
               gradient: 0min to 4min, 100% (A), 
                         4min to 15 min, 100% (A) to 30% (A), 
                         After 15 min, 30% (A) 
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 35℃