Ministry of Health, labour and welfare in Japan regulates maximum residue limits for agricultural chemicals in foods. Based on the revision of the "Food Sanitation Law" in 2003, "positive list system" was enacted May 29, 2006. In Food Sanitation Law #1003001 (Oct 3, 2006), section two states "Analytical method with GC/MS for multi-residues" and "Analytical method with LC/MS for multi-residues" in crops and animal products. In the method, acrinathrin and tricyclazole were used as fractionation standards. For the purification of pesticide residuals in animal products, a GPC clean-up column, CLNpak EV-2000 AC is recommended. Fractionation area is depending on the type of samples used for the GPC clean-up.
Sample : 5mL Acrinathrin Tricyclazole
Column : Shodex CLNpak EV-G AC (20.0mmI.D. x 100mm) + EV-2000 AC (20.0mmI.D. x 300mm)
Eluent : Acetone/Cyclohexane=1/4
Flow rate : 5mL/min
Detector : UV(254nm)
Column temp.: 40°C