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Hydantoins (DE-613)

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Five kinds of hidantoins were analyzed using RSpak DE-613 with aqueous eluent. The elution order of the hidantoins is the order of hydrophilicity of R-group.


Sample : Hydantoins, 0.05% each, 3μL
1. 5-Carboxyethyl Hydantoin
2. 5-Hydroxymethyl Hydantoin
3. Hydantoin
4. 5-Methyl Hydantoin
5. 5-Ethyl Hydantoin
Column       : Shodex RSpak DE-613 (6.0mmI.D. x 150mm)
Eluent       : 1/30M Na2HPO4 + 1/30M KH2PO4 aq.
Flow rate    : 2.0mL/min
Detector     : UV(210nm)
Column temp. : 50°C