, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6234 info(at)shodex.de Konrad-Zuse-Platz 3, Munich Mo-Thu 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, Munich   

Product Search

 

Our Shodex HPLC products can be found here.

Overview product description: 

Shodex HPLC columns show a perfect separation performance. A huge variation of separation techniques is available. The separation techniques start with reversed phase, normal phase, HILIC, ion chromatography, ion exchange, ion exclusion, size exclusion chromatography, multi mode chromatography and a lot more.

The separation performance is guaranteed by using different stationary phases, mostly with polymer-based (or copolymers) resins. Polymeric gels show a variation of advantages compared to silica-based stationary phases, like e.g. almost unlimited pH value usage, chemical stability, bleeding reduction of the stationary phase, low base line noise with use of particle sensitive detectors, high theoretical plate numbers, very low adsorptive behavior and as well a good temperature stability.

The variation options of polymeric and/or copolymeric stationary phases in combination with surface modifications and use of functional groups creates a huge variety, which can be hardly found in silica-based gels.

Our production site produces all steps, starting from the gel production itself to the filling of the columns and as well all relevant quality standard tests. With the above mentioned items we're able to do all LC analysis in a very efficient way.

Reversed Phase Chromatography (RP)

  • Separation is based on the partition equilibrium between stationary phase and mobile phase.
  • The polarity of the stationary phase is lower than that of the mobile phase.
  • Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
  • Using the lower polarity mobile phase causes a faster elution.

Video

Hydrophilic Interaction Chromatography (HILIC)

  • Separation is based on hydrophilic interaction.
  • A high polarity stationary phase is used.
  • Typically the mobile phase contains a mixture of organic solvents such as acetonitrile and aqueous solvents (water or buffer).
  • Using the higher polarity mobile phase causes a faster elution.
  • Applicable for the analysis of high polar substances.

Video

Ligand Exchange Chromatography (LEX)

  • Separation is based on differences in analytes’ coordination complex.
  • Stationary phase modified with metal sulfonate complex ion.
  • Works in combination with size exclusion or HILIC modes.

Video

Ion Chromatography (IC)

  • Separation is based on electrostatic interaction (bonding) between the ion exchanger and ionic solutes.
  • Has a relatively small ion exchange capacity.
  • Electrical conductivity detector can be used with low-salt concentration mobile phase.
  • Used mainly for the analysis of inorganic compounds.

Video

 

 

Size Exclusion Chromatography (SEC)

  • Network or pores on the surface of the packing material works as molecular sieve to separate molecules based on their sizes.
  • To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packing gel interaction.
  • The bigger the molecule size, the faster the elution sequence.
  • Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers.

Video

Ion Exclusion Chromatography (IEX) 

  • Separation is based on electrostatic interaction (repulsion) between the ion exchanger and ionic solutes.
  • Dissociated ionic molecules elute faster than non-dissociated forms.
  • Used mainly for the analysis of organic acids.

Ion Exchange Chromatography (IEC)

  • Separation is based on electrostatic interactions between the ion exchanger and ionic solutes.
  • The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest charge differences between the analyte of interest.
  • The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase.

Normal Phase Chromatography (NP)  

  • Separation is based on the partition equilibrium between the stationary phase and the mobile phase.
  • The polarity of the stationary phase is higher than that of the mobile phase.
  • Typically the mobile phase contains a mixture of organic solvents with different polarities such as hexane and isopropanol.
  • Using the higher polarity mobile phase causes a faster elution.

Affinity Chromatography (AFC)

  • Separation is based on adsorption of the analyte to the specific biologically derived ligand pair.
  • Highly selective.
  • A buffer solution with the appropriate pH and ionic strength is selected based on the type of ligand, analytes, and their interaction.
  • Used mainly for the purification and concentration of biological active substances.

Chiral Separation Chromatography (CS)

  • Separation of optical isomers using chiral selectors.
  • Highly selective.

Hydrophobic Interaction Chromatography (HIC)

  • Separation is based on hydrophobic interaction.
  • Hydrophobic functional group is modified on the stationary phase.
  • Adsorption of analytes generally occurs at a high salt concentration and they are released by lowering the salt concentration.
  • Used mainly for the analysis of proteins.

Multimode Chromatography

  • Separation is based on a combination of several methods

Polymeric Molar Mass Standards for Size Exclusion Chromatography (SEC)

  • Pulluan for aqueous molar mass calibration
  • Polymethylmethacrylate (PMMA) for organic solvent molar mass calibration
  • Polystyrene (PS) for organic solvent molar mass calibration

Shodex Detectors

  • Refractive Index Detectors (RI-501, RI-502, RI-504)
  • Degassers (2 & 4 channel)
  • Conductivity Detector (CD-200)