Recovery of Proteins and Enzyme Activity (DEAE-825)
The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.
| Recovery of proteins |
|---|
| Protein | Recovery (%) |
|---|
| Trypsin inhibitor | 96 |
|---|
| β-Lactoglobulin | 100 |
|---|
| Ovalbumin | 101 |
|---|
| Myoglobin | 100 |
|---|
| γ-Globulin | 99 |
|---|
| BSA | 97 |
|---|
| Thyroglobulin | 90 |
|---|
| Recovery of enzyme activity |
|---|
| Enzyme | Recovery (%) |
|---|
| Kallikrein | 92 |
|---|
| Lipoxidase | 96 |
|---|
| Catalase | 94 |
|---|
Sample load : 0.5mg each
Column : Shodex IEC DEAE-825 (8.0mmI.D. x 75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 1min, (A) to (B) 