Columns for Polymer-based Reversed Phase Chr (RP) + Surfactants, detergent - Size exclusion (SEC)omatography (Asahipak GF, ODP2 HP, RSpak RP18, DS, DE, DM, NN, JJ) (G50)

	Product Code	Product Name	Plate Number (TP/column)	Base material	Functional group	Particle size (µm)	Pore size (Å)	Column size (mm) ID x Length	Max. pressure (MPa)	Max. flow rate (mL/min)	Temperature range (°C)	pH range	Max. salt concentration	Shipping solvent	Exclusion limit (Pullulan)	Exclusion limit (Protein)	Manual
	F7600000	Asahipak GF-210 HQ	>19.000	Polyvinyl alcohol		5	180	7.5 x 300	9.0	1.0	10-60	2 to 9	<0.1M	H2O	9000		Please register or log in
	F7600001	Asahipak GF-310 HQ	>19.000	Polyvinyl alcohol		5	400	7.5 x 300	7.0	1.0	10-60	2 to 9	<0.1M	H2O/CH3OH=70/30	40000		Please register or log in
	F7600002	Asahipak GF-510 HQ	>19.000	Polyvinyl alcohol		5	2000	7.5 x 300	6.5	1.0	10-60	2 to 9	<0.1M	H2O/CH3OH=70/30	300000		Please register or log in
	F7600003	Asahipak GF-710 HQ	>11.000	Polyvinyl alcohol		9	10000	7.5 x 300	2.5	1.0	10-60	2 to 9	<0.1M	H2O/CH3OH=70/30	10.000.000 (estimated)		Please register or log in
	F7600004	Asahipak GF-7M HQ	>13.000	Polyvinyl alcohol		9	10000	7.5 x 300	4.5	1.0	10-60	2 to 9	<0.1M	H2O/CH3OH=70/30	10.000.000 (estimated)		Please register or log in
	F6710018	Asahipak GF-1G 7B	(guard column)	Polyvinyl alcohol		9	400	7.5 x 50	-	-	10-60	2 to 9	<0.1M	H2O/CH3OH=70/30	-		Please register or log in
	F7622001	ODP2 HP-4B	>3.500	Polyhydroxymethacrylate	-	5	40	4.6 x 50	15	1.5	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F7622002	ODP2 HP-4D	>10.000	Polyhydroxymethacrylate	-	5	40	4.6 x 150	15	1.5	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F7622005	ODP2 HP-2D	>7.000	Polyhydroxymethacrylate	-	5	40	2.0 x 150	15	0.3	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F7622003	ODP2 HP-4E	>17.000	Polyhydroxymethacrylate	-	5	40	4.6 x 250	15	1.0	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F6714010	ODP2 HPG-4A	(guard column)	Polyhydroxymethacrylate	-	5	-	4.6 x 10	-	-	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F6714011	ODP2 HPG-2A	(guard column)	Polyhydroxymethacrylate	-	5	-	2.0 x 10	-	-	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F7622004	ODP2 HP-2B	>3.000	Polyhydroxymethacrylate	-	5	40	2.0 x 50	12	0.3	20 to 60	3 to 12	< 0.1 M	H2O/CH3CN=55/45			Please register or log in
	F7009000	RSpak RP18-415	>5.000	Styrene divinylbenzene copolymer	(RP)	6	450	4.6 x 150	22	1.5	20 to 50	1 to 13	<0.5M	H2O/CH3CN=5/95			Please register or log in
	F6709558	RSpak RP18-G	(guard column)	Styrene divinylbenzene copolymer	(RP)	6	-	4.6 x 10	-	-	20 to 50	1 to 13	<0.5M	H2O/CH3CN/THF=40/30/30			Please register or log in
	F7001001	RSpak DS-613	>6.500	Styrene divinylbenzene copolymer	(RP)	6	200	6.0 x 150	10	3.0	RT to 80	2 to 12	<0.5M	H2O/CH3CN/THF=30/40/30			Please register or log in
	F7001012	RSpak DS-413	>11.000	Styrene divinylbenzene copolymer	(RP)	3.5	200	4.6 x 150	20	1.0	20 to 50	1 to 13	<0.5M	H2O/CH3CN/THF=40/30/30			Please register or log in
	F6700140	RSpak DS-G	(guard column)	Styrene divinylbenzene copolymer	(RP)	10	-	4.6 x 10	-	-	RT to 80	1 to 13	<0.5M	H2O/CH3CN/THF=30/40/30			Please register or log in
	F7001004	RSpak DE-613	>7.000	Polymethacrylate	(RP)	6	25	6.0 x 150	7	3.0	20 to 70	2 to 12	< 0.5M	H2O			Please register or log in
	F7001005	RSpak DE-413	>11.000	Polymethacrylate	(RP)	4	25	4.6 x 150	15	1.2	20 to 60	2 to 12	< 0.5M	H2O/CH3CN=50/50			Please register or log in
	F7009030	RSpak DE-413L	>17.000	Polymethacrylate	(RP)	4	25	4.6 x 250	18	1.2	20 to 60	2 to 12	< 0.5M	H2O/CH3CN=50/50			Please register or log in
	F6700150	RSpak DE-G 4A	(guard column)	Polymethacrylate	(RP)	10	-	4.6 x 10	-	-	20 to 70	2 to 12	< 0.5M	H2O			Please register or log in
	F7001007	RSpak DE-213	>8.000	Polymethacrylate	(RP)	4	25	2.0 x 150	12	0.4	20 to 60	2 to 12	< 0.5M	H2O/CH3CN=50/50			Please register or log in
	F6700151	RSpak DE-G 2A	(guard column)	Polymethacrylate	(RP)	6	-	2.0 x 10	-	-	20 to 60	2 to 12	< 0.5M	H2O/CH3CN=50/50			Please register or log in
	F7001002	RSpak DM-614	> 4.500	Polyhydroxymethacrylate	(RP)	10	200	6.0 x 150	2.0	2.0	20 to 60	2 to 10	< 0.5 M	5mM H3PO4 aq.			Please register or log in
	F6700160	RSpak DM-G 4A	(guard column)	Polyhydroxymethacrylate	(RP)	12	-	4.6 x 10	-	-	20 to 60	2 to 10	< 0.5 M	5mM H3PO4 aq.			Please register or log in
	F7008140	RSpak NN-814	>9.000	Polyhydroxymethacrylate	Sulfo	10	200	8.0 x 250	2.0	1.5	10 to 50	2 to 12	< 0.5M	0.1 M Sodium phosphate buffer (pH 3.0)			Please register or log in
	F7008150	RSpak NN-614	>4.000	Polyhydroxymethacrylate	Sulfo	10	200	6.0 x 150	1.5	1.0	10 to 50	2 to 12	< 0.5M	0.1 M Sodium phosphate buffer (pH 3.0)			Please register or log in
	F7008160	RSpak NN-414	>6.000	Polyhydroxymethacrylate	Sulfo	10	200	4.6 x 150	1.2	0.5	10 to 50	2 to 12	< 0.5M	0.1 M Sodium phosphate buffer (pH 3.0)			Please register or log in
	F6700510	RSpak NN-G	(guard column)	Polyhydroxymethacrylate	Sulfo	10	-	6.0 x 50	-	-	10 to 50	2 to 12	< 0.5M	0.1 M Sodium phosphate buffer (pH 3.0)			Please register or log in
	F7008240	RSpak JJ-50 4D	>4.500	Polyvinyl alcohol	Quaternary ammonium	5	100	4.6 x 150	9.0	1.2	10 to 60	2 to 10	<0.1M	H2O/CH3CN=40/60			Please register or log in
	F7008220	RSpak JJ-50 2D	>3.500	Polyvinyl alcohol	Quaternary ammonium	5	100	2.0 x 150	9.0	0.3	10 to 60	2 to 10	<0.1M	H2O/CH3CN=40/60			Please register or log in

Columns for Polymer-based Reversed Phase Chr (RP) + Surfactants, detergent - Size exclusion (SEC)omatography

• Calibration Curves:

• Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)

• Guidelines, HowTos, FAQ:

• Guidelines for Shodex Column Selection : Drugs, Metabolites and Chiral Compounds
• Guidelines for Shodex Column Selection : Organic Acids

• Other Applications:

• Vitamin B₁
• Nucleobases (4) (DE-413)
• Amides (5) (DE-413)
• Amides (2) (Comparison of Reversed Phase Columns)
• Nucleobases and Nucleosides (1) (DE-613)
• Polystyrene Standards (21) (GF-HQ : THF, Chloroform and DMF)
• Ketones (2) (DE-413)
• Soyasaponin in Soy Bean (DS-613)
• Brij^® 56 (Polyoxyethylene(10) Cetyl Ether)
• Nonylphenol Ethoxylates (3) (GF-310 4E)
• Gluconic Acids (DE-613)
• Imidazoles
• Standard Proteins (3) (RP18-415)
• Guanine and Tryptophan (DM-614, NN-814 and DE-613)
• Local Anesthetics (2) (DE-413)
• Effects of Sample Load (Pullulan) (OHpak SB-806M HQ)
• Carnitine
• Short Amines (ODP2 HP)
• Degree of POE Polymerization
• Reproducibility in LC/MS Analysis (ODP2 HP)
• Antifebriles (2) (DM-614)
• LC/MS Analysis of Drugs in Control Serum (ODP2 HP)
• Fatty Acid Methyl Esters (DS-413)
• LC/MS Analysis of Drugs in BSA (2) (ODP2 HP)
• Curative Drugs to Eradicate H.Pyrori
• LC/MS Analysis of Drugs in BSA (1) (ODP2 HP)
• Whey Proteins
• Influence of Proteins in LC/MS Analysis (ODP2 HP-2B)
• Kojic Acid
• Furfural and Guaiacol
• Organic Acid Standards (5) (DE-213)
• Comparison with ODS Column (3)
• Bisphenol A (DE-413)
• LC/MS Analysis of Barbital in BSA (ODP2 HP-2B)
• Sodium Polyoxyethylene Alkyl Ether Sulfate
• Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)
• Elution Volume of Aromatic Organic Acids (NN-814)
• Selection of Column (3) (Poly(Sodium Methacrylate))
• Amino Acids (2) (NN-814)
• Selection of Column (2) (Sodium Polyacrylate)
• Comparison with ODS Column (1)
• Selection of Column (1) (Sodium Poly(Styrene Sulfonate))
• Effects of Organic Solvent Concentration (2)
• Poly(Sodium Methacrylate) (GF-710 HQ)
• Nonylphenol Ethoxylates (1) (GF-310 HQ)
• Pullulan Standards (3) (GF-710 HQ)
• Aliphatic Alcohols (2) (DE-613)
• N-Methoxymethyl Nylon (Methanol Eluent)
• Procainamides (1) (DE-613)
• Influence of Column Temperature on Retention Time of PEG (GF-310 HQ and GS-320 HQ)
• Hydantoins
• Pullulan Standards (2) (GF-310 HQ)
• Citric Acid in Phosphoric Acid (DE-613 + KC-811)
• Recovery of Proteins (GF-310 HQ and GS-320 HQ)
• Analysis of Fatty Acids with Corona CAD (DS-613)
• Poly(o-Cresyl Glycidyl Ether)-co-Formaldehyde (GF-310 HQ)
• Antifebriles (1) (DS-613)
• Adrenal Cortical Hormones (2) (GF-310 HQ)
• Tryptic Digest of Myoglobin (1) (RP18-415)
• Microchemical Analysis of Cationic Surfactants
• Effects of Sample Load on Theoretical Plate Number
• Trimethylalkylammonium Chlorides
• Sulfanilamides (DE-413)
• Benzalkonium Chloride (1) (GF-310 HQ)
• Nonylphenol Ethoxylates (2) (GF-310 4E)
• Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column
• Antibiotics (2) (DE-413)
• Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent
• Procainamides (2) (DE-413)
• Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)
• Meglumine (NN-414)
• Comparison of Chromatograms of Protein mixtures
• Mixture of Nonylphenoxy Carboxylic Acids
• Recovery of Proteins (GF-510 HQ and GS-520 HQ)
• Total Cyanide in River Water
• Standard Proteins (23) (GF-510 HQ)
• Camphor and Benzoic Acid (NN-814)
• Triton X-100 (GF-310 HQ)
• Nucleobases and Nucleosides (2) (NN-814)
• Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
• Water-Soluble Vitamins (3) (NN-814)
• Sodium Alkyl Sulfates
• Permanent Wave Solution (NN-814)
• Analysis of Anticonvulsant in Serum (ODP2 HP)
• Comparison with ODS Column (2)
• Sodium Dodecylbenzenesulfonate (2)
• Effects of Temperature
• Enalapril Maleate (DS-413)
• Effects of Organic Solvents
• Amides (4) (DE-413)
• Effects of Organic Solvent Concentration (1)
• Amides (3) (DE-413)
• Brij^® 30 (Polyoxyethylene(4) Lauryl Ether) (1)
• Lactic acid and Acrylic acid (DE-413)
• Water-Soluble Vitamins (4)
• Di-carboxylic Acids (DE-413)
• Diols
• Organic Acid Standards (4) (DE-413)
• Dipeptides
• Fatty Acids (3) (DE-413)
• Catecholamines (1) (DE-613)
• Nutrient Beverage
• Diuretics (2) (DE-613)
• Food Containing Vitamins (DE-413)
• Aromatic Alcohols
• Water-Soluble Vitamins (1)
• Poly(Ethylene Glycol) Standards (6) (DE-613)
• Aldehydes
• Organic Acid in Phosphoric Acid (DE-613 + KC-811)
• Insecticides (Neonicotinoids) Analysis (DE-413)
• Ginsenoside (Ginseng Saponin) (DS-413)

Applications for Asahipak GF-210 HQ

• Analysis of Various Tween Type Surfactants (GF-210 HQ)