
Asahipak NH2P-50 Säulen
mit Aminogruppen
Die robuste HILIC-Säule für viele allgemeine Anwendungen
Produktinformation
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Geeignet für die Saccharidanalyse im HILIC-Modus
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Packungsmaterial auf Polymerbasis bietet eine hervorragende chemische Stabilität und minimale Verschlechterung über einen längeren Zeitraum
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Aminogruppen
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pH-Stabilität: 2 bis 13 (silanolfrei)
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Lässt sich durch Spülen mit einer alkalischen Lösung leicht regenerieren
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Geeignet für Evaporative Light Scattering Detector (ELSD), Corona Charged Aerosol Detector (CAD) und Massenspektrometrie (LC-MS/MS).
Eluentenbedingungen für Asahipak NH2P-50:
HILIC-Modus
(1) Als Elutionsmittel können Wasser, Acetonitril und Ethanol, entweder allein oder in Mischungen in jedem beliebigen Verhältnis verwendet werden. Bei wässrigen Ethanollösungen sollte die Durchflussrate aufgrund ihrer relativ hohen Viskosität geringer als normal sein.
(2) Verschiedene Arten von Puffern, die in Acetonitril oder Ethanol löslich sind, können zusammen mit den oben genannten Komponenten verwendet werden, sofern im Eluenten kein feststellbarer Niederschlag auftritt.
(3) Es können Eluenten mit einem pH-Wert von 2 bis 13 verwendet werden.
Anionenaustauschmodus
(1) Es können Pufferlösungen wie Phosphat, Acetat und Tris mit oder ohne NaCl, KCl oder Na2SO4 verwendet werden.
(2) Es können Eluenten mit einem pH-Wert von 2 bis 13 verwendet werden.
Das Packungsmaterial für Asahipak NH2P-50 Säulen enthält Aminogruppen, die an schwache Anionenaustauscherharze gebunden sind. Die Aminogruppen liegen im Gleichgewicht (Verhältnis der freien Basen) des freien Typs und des sauren Typs vor.
Der Gleichgewichtszustand hängt vom pH-Wert und der Ionenzusammensetzung des Eluenten ab. Die Elutionszeiten wurden kürzer und die Peakformen wurden schärfer, wenn das Verhältnis der freien Basen mit steigendem pH-Wert zunimmt.

Produktübersicht
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F7630006 | Asahipak NH2P-50 2D | HILIC | Amino | Polyvinyl alcohol | ≥ 3,500 | 5 µm | 100 Å | 2.0 x 150 mm | steel |
F7630010 | Asahipak NH2P-40 2E | HILIC | Amino | Polyvinyl alcohol | ≥ 7,000 | 4 µm | 100 Å | 2.0 x 250 mm | steel |
F6713000 | Asahipak NH2P-50G 2A | HILIC | Amino | Polyvinyl alcohol | (guard) | 5 µm | - | 2.0 x 10 mm | steel |
F7630007 | Asahipak NH2P-40 3E | HILIC | Amino | Polyvinyl alcohol | ≥ 8,500 | 4 µm | 100 Å | 3.0 x 250 mm | steel |
F6710030 | Asahipak NH2P-50G 3A | HILIC | Amino | Polyvinyl alcohol | (guard) | 5 µm | - | 3.0 x 10 mm | steel |
F7630005 | Asahipak NH2P-50 4B | HILIC | Amino | Polyvinyl alcohol | ≥ 1,500 | 5 µm | 100 Å | 4.6 x 50 mm | steel |
F7630002 | Asahipak NH2P-50 4D | HILIC | Amino | Polyvinyl alcohol | ≥ 5,500 | 5 µm | 100 Å | 4.6 x 150 mm | steel |
F7630001 | Asahipak NH2P-50 4E | HILIC | Amino | Polyvinyl alcohol | ≥ 7,500 | 5 µm | 100 Å | 4.6 x 250 mm | steel |
F6710016 | Asahipak NH2P-50G 4A | HILIC | Amino | Polyvinyl alcohol | (guard) | 5 µm | - | 4.6 x 10 mm | steel |
F6830001 | Asahipak NH2P-50 10E | HILIC | Amino | Polyvinyl alcohol | ≥ 10,000 | 5 µm | 100 Å | 10.0 x 250 mm | steel |
F6830031 | Asahipak NH2P-90 20F | HILIC | Amino | Polyvinyl alcohol | ≥ 10,000 | 9 µm | 100 Å | 20.0 x 300 mm | steel |
F6710017 | Asahipak NH2P-130G 7B | HILIC | Amino | Polyvinyl alcohol | (guard) | 13 µm | - | 7.5 x 50 mm | steel |
Produktdetails
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Shipping solvent | USP |
|---|---|---|---|---|---|---|---|
Asahipak NH2P-50 2D | 10 MPa (100 bar) | 0.1 to 0.2 mL/min | 0.4 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-40 2E | 20 MPa (200 bar) | 0.1 to 0.2 mL/min | 0.4 mL/min | 15 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50G 2A | - | - | - | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-40 3E | 13 MPa (130 bar) | 0.30 to 0.35 mL/min | 0.5 mL/min | 15 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50G 3A | - | - | - | 15 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50 4B | 5.0 MPa (50 bar) | 0.3 to 0.6 mL/min | 1.5 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50 4D | 10 MPa (100 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50 4E | 15 MPa (150 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50G 4A | - | - | - | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-50 10E | 15 MPa (150 bar) | 2.0 to 3.0 mL/min | 5.0 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-90 20F | 10 MPa (100 bar) | 5.0 to 10.0 mL/min | 12.0 mL/min | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Asahipak NH2P-130G 7B | - | - | - | 4 to 50°C | 2 to 13 | CH3CN | L82 |
Produkte
Dokumente herunterladen
Shodex Technical Article 11_HPLC Analysis of Antidiabetic Drugs (pdf)
Shodex Technical Article 14_Analysis of Functional Ingredients in Dietary Supplement (pdf)
Bedienungsanleitungen:
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Anwendungen
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Asahipak NH2P-50 4.6 mm ID
Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
Analysis of Carboplatin (NH2P-50 4E)
Analysis of Cyclodextrins (NH2P-50 4E)
Analysis of Dextrose and Fructose in Cranberry Fruit Juice (NH2P-50 4E)
Analysis of Four Saccharides in Honey According to Chinese Pharmacopoeia 2025 Edition (NH2P‑50 4E)
Analysis of GABA in a Supplement (NH2P-50 4E)
Analysis of Galactooligosaccharides According to Ingredient Standards for FOSHU (KS-801, NH2P-50 4E)
Analysis of Imidazole Dipeptides (NH2P-50 4E)
Analysis of Lactobionic Acid (NH2P-50 4E)
Analysis of Levocarnitine (NH2P-50 4E)
Analysis of Maltobionic Acid in Yogurt Drink (NH2P-50 4E)
Analysis of Melamine (1) (NH2P-50 4E)
Analysis of Miglitol According to JP Method (NH2P-50 4E)
Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)
Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
Analysis of Saccharides in Coconut Water (NH2P-50 4E)
Analysis of Sorbitol in Nicardipine Hydrochloride Injection (NH2P-50 4E)
Analysis of Theanine (NH2P-50 4E)
Analysis of Theanine in Supplement (NH2P-50 4E)
Analysis of Voglibose According to JP Method (NH2P-50 4E)
Analysis of Zanamivir According to USP-NF Method (NH2P-50 4E)
Analysis of α-GPC in Supplements (NH2P-50 4E)
Anti-diabetes Drugs (NH2P-50 4E)
Calibration Curves for Saccharides (NH2P-50 4E)
Chitosan-Oligosaccharides (Chitooligosaccharides) (1) (NH2P-50 4E)
Chitosan-Oligosaccharides (Chitooligosaccharides) (2) (Asahipak NH2P-50 4E)
Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
Comparison of Amino Column with Amide Column
Comparison of NH2P with Silica-based Amino Column (1)
Comparison of NH2P with Silica-based Amino Column (2)
Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD
Degradation Product of Cellulose with Cellulase (PcCel45A) Digestion (NH2P-50 4E)
Durability Against Acidic Solvents (NH2P-50 4E)
Durability Against Alkaline Solvents (NH2P-50 4E)
Durability Against Eluent Composition Change (NH2P-50 4E)
Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
Effects of Acetonitrile Concentration on Plate Number (NH2P-50 4E)
Effects of Free Base Ratio of Amino Group on Plate Number (NH2P-50 4E)
Effects of Organic Solvent in Eluent (NH2P-50 4E)
Effects of Sample Injection Volume on Sugar Separation (NH2P-50 4E)
Effects of Sample Preparation Solution on Sugar Separation (NH2P-50 4E)
Elution Volume of Saccharides and Sugar Alcohols (NH2P-50 4E)
Extract of Wheat Rod (NH2P-50 4E)
Fructooligosaccharide Syrup (NH2P-50 4E)
Ginsenoside (Carrot Saponin) (NH2P-50 4E)
Glucose Derivatives (NH2P-50 4E)
High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
Hydrolyzed Dextran (3) (NH2P-50 4E)
Isomaltooligosaccharides (NH2P-50 4E)
Maltooligosaccharides (NH2P-50)
Maltose and Nigerose (NH2P-50 4E)
Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
Monosaccharides (1) (NH2P-50 4E)
Monosaccharides (2) (NH2P-50 4E)
Monosaccharides and Disaccharides (2) (NH2P-50 4E)
N-Acetylchitooligosaccharides (1) (NH2P-50 4E)
Oligogalacturonic Acids (NH2P-50 4E)
Phosphorylated Saccharides (1) (NH2P-50 4E)
Phosphorylated Saccharides (2) (NH2P-50 4E)
Roast Sweet Potato (NH2P-50 4E)
Saccharides Analysis using a Charged Aerosol Detector (NH2P-50 4E)
Saccharides and Amino Sugars (2) (NH2P-50 4E)
Saccharides and Sugar Alcohols (6) (NH2P-50)
Saccharides in Wood (3) (NH2P-50 4E)
Separation of Fosfomycin and its Thermal Decomposition Products (NH2P-50 4E)
Separation of Nitrite and Nitrate Ions (NH2P-50 4E)
Separation of Taurine and Inositol (NH2P-50 4E)
Soft Drink Containing Erythritol (NH2P-50 4E)
Soft Drink Containing Vitamin C (NH2P-50 4E)
Stevioside and Rebaudioside A (NH2P-50 4E)
Water-Soluble Vitamins (5) (NH2P-50 4E)
Yogurt: Sugar added (NH2P-50 4E)
Analysis of L-Rhamnose According to JSFA Method (NH2P-50 4D)
Comparison of Analysis of Glucosamine Hydrochloride between Polymer-based Amino Columns and Silica-based Amino Columns
Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)
Asahipak NH2P-50 3.0 mm ID
Analysis of Trisaccharides (1) (NH2P-40 3E)
Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
Asahipak NH2P-50 2.0 mm ID
Analysis of Melamine (2) (NH2P-50 2D)
LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
LC/MS Analysis of Citrulline (NH2P-40 2D)
LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)
LC/MS Analysis of GABA (NH2P-40 2D)
LC/MS Analysis of Melamine and Cyanuric Acid (NH2P-40 2D)
LC/MS Analysis of Metformin (NH2P-40 2D)
LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)
LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)
LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)
LC/MS Analysis of Various Sweeteners (NH2P-40 2D)
LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)
LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
LC/MS/MS Monitoring of the Estradiol Metabolism (NH2P-40 2D)
LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)
Simultaneous Analysis of Sweeteners and Amino Acids in Energy Drink (NH2P-40 2D)
Asahipak NH2P-50 preparative











