Product information

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Multi mode chromatography is an analytical method combining several separation modes. 

When a single separation mode is not enough for separating the target substance, an improvement can be expected by using multi mode chromatography. The balance between the separation modes involved can be controlled by changing the eluent composition.

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Asahipak GS series [multi-mode columns]

• Polymer-based columns

• SEC is the main separation mode

• With the choice of eluent, the column provides multimode features of reversed phase, HILIC, and ion exchange modes to SEC

• Suitable for the separation of peptides or nucleic acids with similar molecular weights

• Suitable for desalting samples or substituting buffer in protein analysis

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The base material is a polymer, resulting in the following advantages;

1) usable at a wide pH range, 2) washable with alkaline solutions, 3) column durability.

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Asahipak GS columns operate basically in SEC mode. However, depending on the eluent conditions, it allows separation by multiple modes combining reversed phase and ion exchange modes. The column is particularly effective in analyzing samples such as proteins and nucleic acid components, which are made up of both acidic and basic components. In this separation mode, samples are eluted in the order of acidic, neutral and basic. Separation can also be adjusted by changing the eluent components.

1) When a neutral/acidic eluent is used.

Acidic samples: As it is the ion exclusion mode which is in operation, substances are eluted relatively quickly.

Neutral samples: As substances are separated by reversed phase mode, they are eluted moderately.

Basic samples: As it is the ion exchange mode which is in operation, substances are eluted relatively slow.

2) When a basic eluent is used.

Acidic samples: Basically, substances are excluded together at the V(0) position but, with acidic samples of large molecular weights, there are cases in which substances are retained in the column.

Neutral samples: Substances are separated by reversed phase mode.

Basic samples: If the eluent has a higher pH than the pKa of the sample, substances are separated by reversed phase mode as the dissociation of basic samples is suppressed.