
Asahipak GS
columns
separation by size for a variety of molecular weights
The polymer-based SEC (GFC) columns with additional separation mode
Product information
Asahipak GS series [multi-mode columns]
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Polymer-based columns
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SEC is the main separation mode
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With the choice of eluent, the column provides multimode features of reversed phase, HILIC, and ion exchange modes to SEC
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Suitable for the separation of peptides or nucleic acids with similar molecular weights
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Suitable for desalting samples or substituting buffer in protein analysis
Asahipak GS columns operate basically in SEC mode. However, depending on the eluent conditions, it allows separation by multiple modes combining reversed phase and ion exchange modes. The column is particularly effective in analyzing samples such as proteins and nucleic acid components, which are made up of both acidic and basic components. In this separation mode, samples are eluted in the order of acidic, neutral and basic. Separation can also be adjusted by changing the eluent components.
1) When a neutral/acidic eluent is used.
Acidic samples: As it is the ion exclusion mode which is in operation, substances are eluted relatively quickly.
Neutral samples: As substances are separated by reversed phase mode, they are eluted moderately.
Basic samples: As it is the ion exchange mode which is in operation, substances are eluted relatively slow.
2) When a basic eluent is used.
Acidic samples: Basically, substances are excluded together at the V(0) position but, with acidic samples of large molecular weights, there are cases in which substances are retained in the column.
Neutral samples: Substances are separated by reversed phase mode.
Basic samples: If the eluent has a higher pH than the pKa of the sample, substances are separated by reversed phase mode as the dissociation of basic samples is suppressed.
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
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F7600005 | Asahipak GS-220 HQ | SEC | - | Polyvinyl alcohol | ≥ 19,000 | 6 µm | 150 Å | 7.5 x 300 mm | steel |
F7600006 | Asahipak GS-320 HQ | SEC | - | Polyvinyl alcohol | ≥ 19,000 | 6 µm | 400 Å | 7.5 x 300 mm | steel |
F6710019 | Asahipak GS-2G 7B | SEC | - | Polyvinyl alcohol | (guard) | 9 µm | - | 7.5 x 50 mm | steel |
F6810034 | Asahipak GS-220 20G | SEC | - | Polyvinyl alcohol | ≥ 14,000 | 13 µm | 150 Å | 20 x 500 mm | steel |
F6810035 | Asahipak GS-320 20G | SEC | - | Polyvinyl alcohol | ≥ 14,000 | 13 µm | 400 Å | 20 x 500 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP | MW range | Exclusion limit |
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Asahipak GS-220 HQ | 6 MPa (60 bar) | 0.4 to 0.6 mL/min | 1.0 mL/min | 4 to 60°C | 2 to 9 | ≤ 0.5 M | H2O/CH3OH = 70/30 |