Shodex Asahipak GS and GF Series_43.jpg

Asahipak GS 
columns

separation by size for a variety of molecular weights

The polymer-based SEC (GFC) columns with additional separation mode

Download the product overview (pdf)

Product information

 

Asahipak GS series [multi-mode columns]

  • Polymer-based columns

  • SEC is the main separation mode

  • With the choice of eluent, the column provides multimode features of reversed phase, HILIC, and ion exchange modes to SEC

  • Suitable for the separation of peptides or nucleic acids with similar molecular weights

  • Suitable for desalting samples or substituting buffer in protein analysis

Asahipak GS columns operate basically in SEC mode. However, depending on the eluent conditions, it allows separation by multiple modes combining reversed phase and ion exchange modes. The column is particularly effective in analyzing samples such as proteins and nucleic acid components, which are made up of both acidic and basic components. In this separation mode, samples are eluted in the order of acidic, neutral and basic. Separation can also be adjusted by changing the eluent components.

1) When a neutral/acidic eluent is used.

Acidic samples: As it is the ion exclusion mode which is in operation, substances are eluted relatively quickly.

Neutral samples: As substances are separated by reversed phase mode, they are eluted moderately.

Basic samples: As it is the ion exchange mode which is in operation, substances are eluted relatively slow.


2) When a basic eluent is used.

Acidic samples: Basically, substances are excluded together at the V(0) position but, with acidic samples of large molecular weights, there are cases in which substances are retained in the column.

Neutral samples: Substances are separated by reversed phase mode.

Basic samples: If the eluent has a higher pH than the pKa of the sample, substances are separated by reversed phase mode as the dissociation of basic samples is suppressed.

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F7600005
Asahipak GS-220 HQ
SEC
-
Polyvinyl alcohol
≥ 19,000
6 µm
150 Å
7.5 x 300 mm
steel
F7600006
Asahipak GS-320 HQ
SEC
-
Polyvinyl alcohol
≥ 19,000
6 µm
400 Å
7.5 x 300 mm
steel
F6710019
Asahipak GS-2G 7B
SEC
-
Polyvinyl alcohol
(guard)
9 µm
-
7.5 x 50 mm
steel
F6810034
Asahipak GS-220 20G
SEC
-
Polyvinyl alcohol
≥ 14,000
13 µm
150 Å
20 x 500 mm
steel
F6810035
Asahipak GS-320 20G
SEC
-
Polyvinyl alcohol
≥ 14,000
13 µm
400 Å
20 x 500 mm
steel
F6710021
Asahipak GS-20G 7B
SEC
-
Polyvinyl alcohol
(guard)
20 µm
-
7.5 x 50 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
MW range
Exclusion limit
Asahipak GS-220 HQ
6 MPa (60 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 3,000
7,000
Asahipak GS-320 HQ
5 MPa (50 bar)
0.4 to 0.6 mL/min
1.0 mL/min
4 to 60°C
2 to 12
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 20,000
40,000
Asahipak GS-2G 7B
-
-
-
4 to 60°C
2 to 12
≤ 0.5 M
H2O/CH3OH = 70/30
-
-
-
Asahipak GS-220 20G
MPa
0.4 to 0.6 mL/min
8.0 mL/min
4 to 60°C
2 to 9
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 3,000
7,000
Asahipak GS-320 20G
MPa
5.0 to 8.0 mL/min
12 mL/min
4 to 60°C
2 to 12
≤ 0.5 M
H2O/CH3OH = 70/30
-
300 - 20,000
40,000
Asahipak GS-20G 7B
-
-
-
4 to 60°C
2 to 12
≤ 0.5 M
H2O/CH3OH = 70/30
-
-
-

Product info