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Shodex Asahipak NH2P Series_43.jpg

Asahipak NH2P-50 columns

with amino groups

The robust HILIC column for many general applications

Download the product overview (pdf)

Product information

  • Suitable for saccharide analysis using HILIC mode

  • Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period

  • Amino groups

  • pH stability: 2 to 13 (silanol free)

  • Easily regenerated by washing in an alkaline solution

  • Appropriate for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)

Eluent conditions for Asahipak NH2P-50:


HILIC mode
(1) Water, acetonitrile, and ethanol, either alone or in mixtures of any ratio, may be used as eluent. With aqueous ethanol solutions, the flow rate should be lower than normal, because of their relatively high viscosity.
(2) Various types of buffers soluble in acetonitrile or ethanol may be used together with the above components, as long as no observable precipitation occurs in the eluent.
(3) Eluents of pH 2 to 13 can be used.

 

Anion-exchange mode
(1) Buffer solutions such as phosphate, acetate, and tris, with or without NaCl, KCl, or Na2SO4, may be used.
(2) Eluents of pH 2.0 to 13.0 can be used.

The packing material for Asahipak NH2P-50 columns has amino groups which are bonded to weak anion exchange resins. The amino groups exist in the equilibrium (free base ratio) of free type and acid type.

 

The state of the equilibrium depends on pH and ionic composition of the eluent. The elution times became shorter and peak shapes became sharper as free base ratio increased with higher pH.

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Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F7630006
Asahipak NH2P-50 2D
HILIC
Amino
Polyvinyl alcohol
≥ 3,500
5 µm
100 Å
2.0 x 150 mm
steel
F7630010
Asahipak NH2P-40 2E
HILIC
Amino
Polyvinyl alcohol
≥ 7,000
4 µm
100 Å
2.0 x 250 mm
steel
F6713000
Asahipak NH2P-50G 2A
HILIC
Amino
Polyvinyl alcohol
(guard)
5 µm
-
2.0 x 10 mm
steel
F7630007
Asahipak NH2P-40 3E
HILIC
Amino
Polyvinyl alcohol
≥ 8,500
4 µm
100 Å
3.0 x 250 mm
steel
F6710030
Asahipak NH2P-50G 3A
HILIC
Amino
Polyvinyl alcohol
(guard)
5 µm
-
3.0 x 10 mm
steel
F7630005
Asahipak NH2P-50 4B
HILIC
Amino
Polyvinyl alcohol
≥ 1,500
5 µm
100 Å
4.6 x 50 mm
steel
F7630002
Asahipak NH2P-50 4D
HILIC
Amino
Polyvinyl alcohol
≥ 5,500
5 µm
100 Å
4.6 x 150 mm
steel
F7630001
Asahipak NH2P-50 4E
HILIC
Amino
Polyvinyl alcohol
≥ 7,500
5 µm
100 Å
4.6 x 250 mm
steel
F6710016
Asahipak NH2P-50G 4A
HILIC
Amino
Polyvinyl alcohol
(guard)
5 µm
-
4.6 x 10 mm
steel
F6830001
Asahipak NH2P-50 10E
HILIC
Amino
Polyvinyl alcohol
≥ 10,000
5 µm
100 Å
10.0 x 250 mm
steel
F6830031
Asahipak NH2P-90 20F
HILIC
Amino
Polyvinyl alcohol
≥ 10,000
9 µm
100 Å
20.0 x 300 mm
steel
F6710017
Asahipak NH2P-130G 7B
HILIC
Amino
Polyvinyl alcohol
(guard)
13 µm
-
7.5 x 50 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Shipping solvent
USP
Asahipak NH2P-50 2D
10 MPa (100 bar)
0.1 to 0.2 mL/min
0.4 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-40 2E
20 MPa (200 bar)
0.1 to 0.2 mL/min
0.4 mL/min
15 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50G 2A
-
-
-
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-40 3E
13 MPa (130 bar)
0.30 to 0.35 mL/min
0.5 mL/min
15 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50G 3A
-
-
-
15 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50 4B
5.0 MPa (50 bar)
0.3 to 0.6 mL/min
1.5 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50 4D
10 MPa (100 bar)
0.5 to 1.0 mL/min
1.5 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50 4E
15 MPa (150 bar)
0.5 to 1.0 mL/min
1.5 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50G 4A
-
-
-
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-50 10E
15 MPa (150 bar)
2.0 to 3.0 mL/min
5.0 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-90 20F
10 MPa (100 bar)
5.0 to 10.0 mL/min
12.0 mL/min
4 to 50°C
2 to 13
CH3CN
L82
Asahipak NH2P-130G 7B
-
-
-
4 to 50°C
2 to 13
CH3CN
L82

Products

Applications

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Analysis of α-GPC in Supplements (NH2P-50 4E)

Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)

Analysis of Carboplatin (NH2P-50 4E)

Analysis of Cyclodextrins (NH2P-50 4E)

Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)

Analysis of GABA Supplement (NH2P-50 4E)

Analysis of Imidazole Dipeptides (NH2P-50 4E)

Analysis of Levocarnitine (NH2P-50 4E)

Analysis of Miglitol According to JP Method (NH2P-50 4E)

Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)

Analysis of Pyridylaminated (PA) Monosaccharides

Analysis of Pyridylaminated Sugar Chains

Analysis of Sorbitol in Nicardipine Hydrochloride Injection (NH2P-50 4E)

Analysis of Theanine (NH2P-50 4E)

Analysis of Theanine in Supplement (NH2P-50 4E)

Analysis of Trisaccharides (1) (NH2P-40 3E)

Analysis of Voglibose According to JP Method (NH2P-50 4E)

Analysis of Zanamivir According to USP-NF Method (NH2P-50 4E)

Anti-diabetes Drugs (NH2P-50 4E)

Apple Juice (NH2P-50 4E)

Betaine (NH2P-50 4E)

Calibration Curves for Saccharides (NH2P-50 4E)

Chitosan-Oligosaccharides (Chitooligosaccharides) (1) (NH2P-50 4E)

Chitosan-Oligosaccharides (Chitooligosaccharides) (2) (Asahipak NH2P-50 4E)

Chocolate Cake (NH2P-50)

Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)

Comparison of Amino Column with Amide Column

Comparison of Analysis of Glucosamine Hydrochloride between Polymer-based Amino Columns and Silica-based Amino Columns

Comparison of NH2P with Silica-based Amino Column (1)

Comparison of NH2P with Silica-based Amino Column (2)

Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD

Degradation Product of Cellulose with Cellulase (PcCel45A) Digestion

Durability Against Acidic Solvents (NH2P-50 4E)

Durability Against Alkaline Solvents (NH2P-50 4E)

Durability Against Eluent Composition Change (NH2P-50 4E)

Effects of Acetonitrile Concentration on Elution Time (NH2P-50)

Effects of Acetonitrile Concentration on Plate Number

Effects of Free Base Ratio of Amino Group on Plate Number

Effects of Organic Solvent Concentration on HILIC Mode (NH2P-50 4B)

Effects of Organic Solvent in Eluent (NH2P-50 4E)

Effects of Sample Injection Volume (NH2P-50 4E)

Effects of Sample Solvent Composition (NH2P-50 4E)

Elution Volume of Saccharides

Elution Volume of Saccharides and Sugar Alcohols (NH2P-50 4E)

Extract of Wheat Rod (NH2P-50 4E)

Fructooligosaccharide Syrup (NH2P-50 4E)

Galactooligosaccharides (2) (KS-801, NH2P-50 4E)

Ginsenoside (Carrot Saponin) (NH2P-50 4E)

Glucose Derivatives (NH2P-50 4E)

High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)

Hydrolyzed Dextran (3) (NH2P-50 4E)

Isomaltooligosaccharides (NH2P-50 4E)

Jelly (NH2P-50 4E)

LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)

LC/MS Analysis of Citrulline (NH2P-40 2D)

LC/MS Analysis of Essential Amino Acids (NH2P-40 2D)

LC/MS Analysis of GABA (NH2P-40 2D)

LC/MS Analysis of Melamine and Cyanuric Acid (NH2P-40 2D)

LC/MS Analysis of Metformin (NH2P-40 2D)

LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)

LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)

LC/MS Analysis of Tranexamic Acid (NH2P-40 2D)

LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)

LC/MS Analysis of Various Sweeteners (NH2P-40 2D)

LC/MS/MS Analysis of Creatine and Related Substances (NH2P-40 2D)

LC/MS/MS Analysis of Saccharides (NH2P-40 2D)

LC/MS/MS Monitoring of the Estradiol Metabolism (NH2P-40 2D)

LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG)

Maltooligosaccharides (NH2P-50)

Maltose and Nigerose (NH2P-50 4E)

Melamine (1) (NH2P-50 4E)

Melamine (2) (NH2P-50 2D)

Milk Coffee (NH2P-50 4E)

Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)

Monosaccharides (1) (NH2P-50 4E)

Monosaccharides (2) (NH2P-50 4E)

Monosaccharides and Disaccharides (2) (NH2P-50 4E)

Monosaccharides and Disaccharides (3) (NH2P-50 4E)

N-Acetylchitooligosaccharides (1) (NH2P-50 4E)

Oligogalacturonic Acids (NH2P-50 4E)

Phosphorylated Saccharides (1) (NH2P-50 4E)

Phosphorylated Saccharides (2) (NH2P-50 4E)

Roast Sweet Potato (NH2P-50 4E)

Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)

Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)

Saccharides and Amino Sugars (2) (NH2P-50 4E)

Saccharides and Sugar Alcohols (6) (NH2P-50)

Saccharides in Wood (3) (NH2P-50 4E)

Separation of Fosfomycin and its thermal decomposition products (NH2P-50 4E)

Separation of Nitrite and Nitrate Ions (NH2P-50 4E)

Separation of Taurine and Inositol (NH2P-50 4E)

Short-Chain Amylose (1) (NH2P-50 10E)

Simultaneous Analysis of Nitrous Acid and Ammonium Thiocyanate According to Japanese Testing Methods for Fertilizers (NH2P-50 4E)

Simultaneous Analysis of Saccharides and Sweeteners in Yogurt Drink (NH2P-40 2D)

Simultaneous Analysis of Sweeteners and Amino Acids in Energy Drink (NH2P-40 2D)

Soft Drink Containing Erythritol (NH2P-50 4E)

Soft Drink Containing Vitamin C (NH2P-50 4E)

Stevioside and Rebaudioside A (NH2P-50 4E)

Sugar Alcohols (2) (NH2P-50)

Vitamin C (3) (NH2P-50 4E)

Water-Soluble Vitamins (5) (NH2P-50 4E)

Xilitol in Tablet Candy (NH2P-50 4E)

Yogurt: Sugar added (NH2P-50)

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