Asahipak NH2P-50 columns

with amino groups

The robust HILIC column for many general applications

Download the product overview (pdf)

Product information

Suitable for saccharide analysis using HILIC mode
Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period
Amino groups
pH stability: 2 to 13 (silanol free)
Easily regenerated by washing in an alkaline solution
Appropriate for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)

Eluent conditions for Asahipak NH2P-50:

HILIC mode
(1) Water, acetonitrile, and ethanol, either alone or in mixtures of any ratio, may be used as eluent. With aqueous ethanol solutions, the flow rate should be lower than normal, because of their relatively high viscosity.
(2) Various types of buffers soluble in acetonitrile or ethanol may be used together with the above components, as long as no observable precipitation occurs in the eluent.
(3) Eluents of pH 2 to 13 can be used.

Anion-exchange mode
(1) Buffer solutions such as phosphate, acetate, and tris, with or without NaCl, KCl, or Na2SO4, may be used.
(2) Eluents of pH 2.0 to 13.0 can be used.

The packing material for Asahipak NH2P-50 columns has amino groups which are bonded to weak anion exchange resins. The amino groups exist in the equilibrium (free base ratio) of free type and acid type.

The state of the equilibrium depends on pH and ionic composition of the eluent. The elution times became shorter and peak shapes became sharper as free base ratio increased with higher pH.

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F7630006	Asahipak NH2P-50 2D	HILIC	Amino	Polyvinyl alcohol	≥ 3,500	5 µm	100 Å	2.0 x 150 mm	steel
F7630010	Asahipak NH2P-40 2E	HILIC	Amino	Polyvinyl alcohol	≥ 7,000	4 µm	100 Å	2.0 x 250 mm	steel
F6713000	Asahipak NH2P-50G 2A	HILIC	Amino	Polyvinyl alcohol	(guard)	5 µm	-	2.0 x 10 mm	steel
F7630007	Asahipak NH2P-40 3E	HILIC	Amino	Polyvinyl alcohol	≥ 8,500	4 µm	100 Å	3.0 x 250 mm	steel
F6710030	Asahipak NH2P-50G 3A	HILIC	Amino	Polyvinyl alcohol	(guard)	5 µm	-	3.0 x 10 mm	steel
F7630005	Asahipak NH2P-50 4B	HILIC	Amino	Polyvinyl alcohol	≥ 1,500	5 µm	100 Å	4.6 x 50 mm	steel
F7630002	Asahipak NH2P-50 4D	HILIC	Amino	Polyvinyl alcohol	≥ 5,500	5 µm	100 Å	4.6 x 150 mm	steel
F7630001	Asahipak NH2P-50 4E	HILIC	Amino	Polyvinyl alcohol	≥ 7,500	5 µm	100 Å	4.6 x 250 mm	steel
F6710016	Asahipak NH2P-50G 4A	HILIC	Amino	Polyvinyl alcohol	(guard)	5 µm	-	4.6 x 10 mm	steel
F6830001	Asahipak NH2P-50 10E	HILIC	Amino	Polyvinyl alcohol	≥ 10,000	5 µm	100 Å	10.0 x 250 mm	steel
F6830031	Asahipak NH2P-90 20F	HILIC	Amino	Polyvinyl alcohol	≥ 10,000	9 µm	100 Å	20.0 x 300 mm	steel
F6710017	Asahipak NH2P-130G 7B	HILIC	Amino	Polyvinyl alcohol	(guard)	13 µm	-	7.5 x 50 mm	steel

Product details

Product name	Maximum pressure	Usual flow rate	Maximum flow rate	Temperature range	pH range	Shipping solvent	USP
Asahipak NH2P-50 2D	10 MPa (100 bar)	0.1 to 0.2 mL/min	0.4 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-40 2E	20 MPa (200 bar)	0.1 to 0.2 mL/min	0.4 mL/min	15 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50G 2A	-	-	-	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-40 3E	13 MPa (130 bar)	0.30 to 0.35 mL/min	0.5 mL/min	15 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50G 3A	-	-	-	15 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50 4B	5.0 MPa (50 bar)	0.3 to 0.6 mL/min	1.5 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50 4D	10 MPa (100 bar)	0.5 to 1.0 mL/min	1.5 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50 4E	15 MPa (150 bar)	0.5 to 1.0 mL/min	1.5 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50G 4A	-	-	-	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-50 10E	15 MPa (150 bar)	2.0 to 3.0 mL/min	5.0 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-90 20F	10 MPa (100 bar)	5.0 to 10.0 mL/min	12.0 mL/min	4 to 50°C	2 to 13	CH3CN	L82
Asahipak NH2P-130G 7B	-	-	-	4 to 50°C	2 to 13	CH3CN	L82

Download documents

Shodex Technical Article 11_HPLC Analysis of Antidiabetic Drugs (pdf)

Shodex Technical Article 14_Analysis of Functional Ingredients in Dietary Supplement (pdf)

English:

Operation Manual Asahipak NH2P-40 2E_20221202E

Operation Manual Asahipak NH2P-40 3E_20221202E

Operation Manual Asahipak NH2P-50 2D_20221202E

Operation Manual Asahipak NH2P-50 4B, 4D, 4E_20221202E

Operation Manual Asahipak NH2P-50 10E_prep_20221202E

Operation Manual Asahipak NH2P-90 20F_prep_20221202E

German:

Bedienungsanleitung Asahipak NH2P-40 2E_20221202G

Bedienungsanleitung Asahipak NH2P-40 3E_20221202G

Bedienungsanleitung Asahipak NH2P-50 2D_20221202G

Bedienungsanleitung Asahipak NH2P-50 4B, 4D, 4E_20221202G

Bedienungsanleitung Asahipak NH2P-50 10E_prep_20221202G

Bedienungsanleitung Asahipak NH2P-90 20F_prep_20221202G

Applications

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Analysis of α-GPC in Supplements (NH2P-50 4E)

Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)

Analysis of Carboplatin (NH2P-50 4E)

Analysis of Cyclodextrins (NH2P-50 4E)

Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)

Analysis of GABA Supplement (NH2P-50 4E)

Analysis of Imidazole Dipeptides (NH2P-50 4E)

Analysis of Levocarnitine (NH2P-50 4E)

Analysis of L-Rhamnose in Accordance with the Japan’s Specifications and Standards for Food Additives (NH2P-50 4D)

Analysis of Miglitol According to JP Method (NH2P-50 4E)

Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)

Analysis of Pyridylaminated (PA) Monosaccharides