RSpak columns
RSpak NN-814 and JJ-50 2D
Polymer-based RP and mixed-mode columns for many different applications
Download the product overview (pdf)
Product information
The RSpak NN-814 and JJ-50 2D combine the reversed phase (RP) mode with ion exchange chromatography (IEC)
RSpak NN-814
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The packing material modified with sulfo groups supports multimode (reversed phase and cation exchange) analysis
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Ideal for the analysis of complex samples containing neutral and ionic substances
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suited for the separation of water-soluble substances such as nucleotides, nucleosides, nucleobases, water-soluble vitamins and amino acids
RSpak JJ-50 2D
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The packing material is modified with trace amounts of quaternary ammonium groups, and supports multimode (reversed phase and anion exchange) analysis
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Ideal for analysis of complex samples containing neutral and ionic substances
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F7008140 | RSpak NN-814 | RP | Sulfo | Polyhydroxy-methacrylate | ≥ 9,000 | 10 µm | 200 Å | 8.0 x 250 mm | steel |
F6700510 | RSpak NN-G | RP | Sulfo | Polyhydroxy-methacrylate | (guard) | 10 µm | 200 Å | 6.0 x 50 mm | steel |
F7008220 | RSpak JJ-50 2D | RP | Quaternary ammonium | Polyvinyl alcohol | ≥ 3,500 | 5 µm | 100 Å | 2.0 x 150 mm | steel |
Product details
Product name | Maximum pressure | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP |
|---|---|---|---|---|---|---|---|
RSpak NN-814 | 2 MPa (20 bar) | 1.5 mL/min | 10 to 50°C | 2 to 12 | ≤ 0.5 M | 0.1 M sodium phosphate buffer (pH 3) | - |
RSpak NN-G | - | - | 10 to 50°C | 2 to 12 | ≤ 0.5 M | 0.1 M sodium phosphate buffer (pH 3) | - |
RSpak JJ-50 2D | 9 MPa (90 bar) | 0.3 mL/min | 10 to 60°C | 2 to 10 | ≤ 0.1 M | H2O/ACN=40/60 | - |
Product info
Reversed Phase - RSpak NN / JJ
Product instruction
Eluents
RSpak NN-814
Following eluents can be used: (1) Buffer, (2) Buffer + salt, (3) Buffer + polar organic solvent, (4) Buffer + salt + polar organic solvent.
The maximum concentration of organic solvents, methanol, ethanol and acetonitrile is 50%.
Separation of the sample can be controlled by changing eluent:
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Water / polar organic solvent
The elution time of substances which have long alkyl chain or benzene ring is rather long. The elution time of such substances can be shorten by raising the concentration of polar organic solvent.
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pH
In the case of basic substances, the lower the eluent pH value, the faster the elution time.
The pH range of eluent should be 2.0 to 12.0. In the case of eluent containing chlorine ions, the pH should be 4.0 or higher.
Strong acids, strong bases and perchloric acid should not be used.
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Salt concentration
In case of basic substances, the higher the salt concentration of the eluent, the faster the elution time.
The maximum concentration of salt is 0.5 M.
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Column temperature
The elution time of ionic substances changes according to the temperature.
RSpak JJ-50 2D
(1) A mixture of water and acetonitrile or water and methanol of any ratio can be used. Use of organic solvents other than acetonitrile or methanol will not be covered under warranty.
(2) Buffers and aqueous solutions of different salts can be used instead of water. They include phosphate, acetate, and Tris buffers and aqueous solutions of sodium chloride, potassium chloride, and sodium sulfate. Please keep total concentration of salts under 0.1 M.
Cleaning/Regeneration
RSpak NN-814
Inject 20 to 50 μL of 2 N nitric acid aqueous solution two or three times.
RSpak JJ-50 2D
(1) Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended flow rate.
(2) Follow below cleaning steps for adsorbing components. For an efficient cleaning, reverse the flow direction and use the flow rate at 0.1 mL/min:
(Pollution by low valency hydrophilic ions)
1) H2O 6 min, 2) 0.1 M NaOH aq. 75 min, 3) H2O 12 min, 4) Eluent 40 min
(Pollution by metal ions)
1) H2O 6 min, 2) 0.1 M HNO3 aq. 75 min, 3) H2O 6 min, 4) 0.1 M NaOH aq. 75 min, 5) H2O 12 min, 6) Eluent 40 min
(Pollution by high valency hydrophobic ions)
1) H2O 25 min, 2) 5 % acetonitrile aq. 20 min, 3) 100 % acetonitrile 100 min, 4) H2O 50 min
Download documents
Column Manual RSpak JJ-50 2D_211124E (pdf)
Column Manual RSpak NN-814_211102E (pdf)
Applications
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RSpak NN-814
Analysis of Meglumine (NN-814)
Camphor and Benzoic Acid (NN-814)
Elution Volume of Aromatic Organic Acids (NN-814)
Elution Volume of Organic Acids (NN-814)
Guanine and Tryptophan (DM-614, NN-814 and DE-613)
Nucleobases and Nucleosides (2) (NN-814)
Organic Acids in Permanent Wave Solution (NN-814)
Permanent Wave Solution (NN-814)