How to connect guard columns

Shodex is offering small complete columns which are used as guard columns.

Guard column names are indicated with "G" in the name.

 

The guard column and the main analytical column can easily be connected by fittings and capillaries. Please use short capillaries with small inner diameter to avoid dead volume.

Picture overview about some examples of classical guard columns from Shodex:

Shodex Asahipak GS-2G 7B
Shodex Asahipak GS-2G 7B

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Shodex Asahipak GS-10G 7B
Shodex Asahipak GS-10G 7B

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Shodex SUGAR KS-G 6B
Shodex SUGAR KS-G 6B

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Shodex Asahipak GS-2G 7B
Shodex Asahipak GS-2G 7B

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​List of classical guard columns:

 

Asahipak GF-1G 7B

Asahipak GS-10G 7B

Asahipak GS-20G 7B

Asahipak GS-2G 7B

Asahipak NH2P-50G 2A

Asahipak NH2P-50G 3A

Asahipak NH2P-50G 4A

Asahipak C4P-50G 4A

Asahipak ODP-50G 2A

Asahipak ODP-50G 4A

Asahipak ODP-50G 6A

CXpak P-G

GPC KD-G 4A

GPC KF-G 4A

GPC LF-G

HILICpak VC-50G 2A

HILICpak VG-50G 2A

HILICpak VG-50G 4A

HILICpak VN-50G 2A

HILICpak VN-50G 4A

HILICpak VT-50G 2A

IC NI-G

IC IA-G

IC SI-90G

IC SI-50G

IC SI-92G

IC SI-95G

IC YS-G

IC YK-G

ODP2 HPG-2A

ODP2 HPG-4A

OHpak LB-G 6B

OHpak SB-807G

OHpak SB-G 6B

OHpak SB-G 8B

PROTEIN KW400G-4A

PROTEIN KW-G 6B

PROTEIN LS-G 4J

PROTEIN LW-G 6B

RSpak DC-G 4A

RSpak DE-G 2A

RSpak DE-G 4A

RSpak DM-G 4A

RSpak DS-G

RSpak KC-G 6B

RSpak KC-G 8B

RSpak NN-G

SUGAR KS-G 6B

SUGAR SC-1211G 4A

SUGAR SC-G 6B

SUGAR SH-G

SUGAR SP-G 6B

SUGAR SZ-G

There are the following exceptions, which consist of a holder and exchangeable filters:

GPC HK-G, IC SI-2GF and line filter IC FL-1.

 

Picture overview about the exceptional guard columns from Shodex, which consist of holder and filters:

Shodex IC SI-2GF | guard holder + filter
Shodex IC SI-2GF | guard holder + filter

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Shodex FL-1 | line filter for IC
Shodex FL-1 | line filter for IC

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GPC HK-G | guard holder + filter
GPC HK-G | guard holder + filter

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Shodex IC SI-2GF | guard holder + filter
Shodex IC SI-2GF | guard holder + filter

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List of exceptional guard columns:

IC SI-2GF (1 holder + 1 filter)

IC SI-2GF filter (3 filters)

This guard column is used for the IC column for anions IC SI-35 2B (2.0 x 50 mm).

IC FL-1 (1 holder + 1 filter)

IC FL-1 filter (5 filters)

This line filter is used in anion and cation Ion Chromatography (IC). It is installed in the eluent line before the injector and removes insoluble components.

GPC HK-G (1 holder + 1 filter)

GPC HK-G filter (3 filters)

This guard column is used for the ultra-rapid SEC column series GPC HK-400 series (4.6 x 150 mm).

Why use guard columns?

Guard columns protect the main analytical column from impurities and matrix from the sample. It prolongs the life time of the analytical column. Usually 2 (sometimes 3, depending on the sample) guard columns are used for the useful life of the main column.

Guard columns can be quickly exchanged. Elevated pressure or broader peak forms can be signs, that the guard column should be checked.

When cleaning the guard column and the main column it is important to disconnect them first and to clean them separately. This prevents the transfer of impurities.

To flush out particles, that might be stuck and accumulated at the inlet frit of the guard column, it is recommended to install it in reverse flow direction and carefully pump eluent at a low flow rate.

-> Guard columns have no manual. The information about the guard columns is included in the manual for the main column.

Where to get column fittings, capillaries or column couplers?

Shodex is not offering HPLC instrument consumables like fittings, ferrules and tubes. Please contact your laboratory supplier.

Fittings, Ferrules and Tubes - Overview for connection of Shodex HPLC guard columns with main columns?

1. Fitting and ferrule types for Shodex columns:

  • Materials: Stainless steel (SUS) or PEEK fittings can be used

  • The size of the screws and ferrules are the same for all Shodex columns, i.e. no difference for micro or conventional type columns.

  • Screw type: unified thread standard No. 10-32 UNF

  • Nut 1/16 (Waters type)

  • Ferrule 1/16 (Waters type)

 


Picture: SUS-based and PEEK-based fitting, ferrule and tube

Fittings SUS PEEK.png

2. Tubing for Shodex Columns:

  • 1/16 outer diameter (O.D.)

  • 0.25 mm inner diameter (I.D.) for analytical columns

  • 0.10 mm inner diameter (I.D.) for semi-micro columns

  • Length of tubing connection depends on the arrangement of columns in the oven:

approx. 10 cm in the case the connection has to be bended
approx. 5 cm in the case the columns can be arranged in line

Picture: bended SUS tube to connect guard column and main column in a short column oven; PEEK tube and fittings in line to connect guard column and main column, when sufficient space is given in the column oven. In both cases the materials can be also switched.

Fittings connection.png

3. Capillary length under the ferrule for Shodex columns:

  • 2.8 mm*: for Asahipak ODP-50, Asahipak NH2P-50, GPC KF-800, OHpak SB-800, SUGAR series

  • 5 mm*: for Asahipak GF-HQ, Asahipak GS-HQ and IEC series


*These values are not guaranteed and should be treated as reference. Since this length is adjusted by the used ferrule for tubing, these values are not strictly fixed.


It is possible to adjust the length of the tubing under the ferrule with PEEK fittings.


How to prepare the SUS tubing and adjust the under ferrule length:

1. Prepare one tube, one screw and one ferrule.

2. Set the screw and the ferrule to the tube. At this time, the ferrule is not fixed.

3. Prepare the column, and release one plug.

4. Set the assembled tube to the column, and tighten the screw by spanner.

5. Release the screw. The ferrule is fixed to the tube.

Picture: examples for capillary length under the ferrule, it depends on the type of column housing.

Fittings length.png