SUGAR KS-800 columns
with Sulfo (Na+) groups
The Ligand Exchange column for mono-, oligo- and poly-saccharides
Product information
SUGAR KS-801 and KS-802
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Separates mono- and oligo-saccharides by combination of ligand exchange (Na+) and size exclusion mode
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Only water is required for the analysis of neutral sugars
KS-803 to 804
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Suitable for separation of polysaccharides by size exclusion mode
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Can be used in combination with other columns e.g., KS-802 and KS-801
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Only water is required for the analysis of neutral sugars
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Fulfill USP L22 and L58 requirements
These columns are mainly used in routine quality controls in food analysis.
This column series has similar properties like the following references:
BioRad Aminex HPX-87N (Na+), Agilent Hi-Plex PL Na, Phenomenex Rezex RNM-Carbohydrate Na+, Sepax Carbomix Na, Thermo Fisher HyperREZ XP Carbyhydrate Na+, Transgenomic Carbo Sep Coregel 87N, CHO-611.
RSpak DC-613
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Separates mono- and oligo-saccharides by combination of ligand exchange (Na+) and HILIC mode
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Water and acetonitrile is used for the analysis
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DC-613 can analyze sugars without removing sodium salts in the sample
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|
F6378010 | SUGAR KS-801 | Ligand Exchange | Sulfo (Na+) | Styrene divinylbenzene copolymer | ≥ 17,000 | 6 µm | 8.0 x 300 mm | steel |
F6378020 | SUGAR KS-802 | Ligand Exchange | Sulfo (Na+) | Styrene divinylbenzene copolymer | ≥ 17,000 | 6 µm | 8.0 x 300 mm | steel |
F6378025 | SUGAR KS-803 | Ligand Exchange | Sulfo (Na+) | Styrene divinylbenzene copolymer | ≥ 17,000 | 6 µm | 8.0 x 300 mm | steel |
F6378035 | SUGAR KS-804 | Ligand Exchange | Sulfo (Na+) | Styrene divinylbenzene copolymer | ≥ 17,000 | 7 µm | 8.0 x 300 mm | steel |
F6700020 | SUGAR KS-G 6B | Ligand Exchange | Sulfo (Na+) | Styrene divinylbenzene copolymer | (guard) | 10 µm | 6.0 x 50 mm | steel |
F7001003 | RSpak DC-613 | Ligand Exchange + HILIC | Sulfo (Na+) | Styrene divinylbenzene copolymer | ≥ 5,500 | 6 µm | 6.0 x 150 mm | steel |
F6700170 | RSpak DC-G 4A | Ligand Exchange + HILIC | Sulfo (Na+) | Styrene divinylbenzene copolymer | (guard) | 10 µm | 4.6 x 10 mm | steel |
Product details
Product code | Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | Shipping solvent | USP | Exclusion limit |
|---|---|---|---|---|---|---|---|---|
F6378010 | SUGAR KS-801 | 5.0 MPa (50 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 20 to 85°C | H2O | L22, L58 | 1,000 |
F6378020 | SUGAR KS-802 | 5.0 MPa (50 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 20 to 85°C | H2O | L22, L58 | 10,000 |
F6378025 | SUGAR KS-803 | 5.0 MPa (50 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 20 to 85°C | H2O | L22, L58 | 50,000 |
F6378035 | SUGAR KS-804 | 5.0 MPa (50 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 20 to 85°C | H2O | L22, L58 | 400,000 |
F6700020 | SUGAR KS-G 6B | - | - | - | 20 to 85°C | H2O | L22, L58 | - |
F7001003 | RSpak DC-613 | 5.0 MPa (50 bar) | 0.5 to 1.0 mL/min | 3.0 mL/min | 40 to 85°C | H2O/ACN = 30/70 | L22, L58 | - |
F6700170 | RSpak DC-G 4A | - | - | - | 40 to 85°C | H2O/ACN = 30/70 | L22, L58 | - |
Applications
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SUGAR KS-800
General
Calibration Curves for KS-800 Series (Aqueous Eluent: Pullulan)
Calibration Curves for KS-800 Series (Column Combination)
Explanation on Ligand Exchange Mode
Guidelines for Shodex Column Selection : by Combination of Saccharides
Guidelines for Shodex Column Selection : by Elution Volume
Guidelines for Shodex Column Selection : by Sample Category
Combination of Columns with Different Counter Ions
Comparison of Columns with Different Counter Ions
Prevention of Anomer Separation
Applications
Analysis of Fructooligosaccharide Components (KS-802 + KS-801)
Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
Analysis of Inositol According to Japanese Pharmaceutical Excipients 2018 (KS-801)
Analysis of Maltose According to USP-NF Method (KS-801)
Analysis of Maltose Hydrate According to JP Method (KS-801)
Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
Analysis of Ribose According to USP-NF Method (KS-801)
Analysis of Saccharides and Furfurals (KS-801)
Analysis of Trehalose According to JP Method (KS-801)
Analysis of Trehalose According to USP-NF Method (KS-801)
Cello-oligosaccharides and Ethanol (KS-802)
Chondroitin Sulfate (KS-804 + KS-801)
Chondroitin Sulfate A (KS-804)
Cyclodextrins (GS-220, KS-802)
Galactooligosaccharides (2) (KS-801, NH2P-50 4E)
Hydrolyzed Dextran (2) (KS-802)
Hydrolyzed Dextran (5) (KS-802)
Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)
Oligosaccharide in Soy Bean (KS-801, KS-802)
Pullulan, Saccharides and Ethanol (KS-802)
Pullulan and Oligosaccharides (KS-804)
Saccharides and Amino Acids (3) (KS-801)
Separation of Kestose and Maltose (KS-802)
Xylooligosaccharides (1) (KS-801)
RSpak DC-613
Calibration curve
SUGAR KS-800 series is suitable to the analysis of polysaccharides. Calibration curves for KS-800 series using pullulan (polysaccahrid standard) are shown here. Besides KS-800 series, OHpak SB-800 HQ series and Asahipak GS-HQ series also can be used for the analysis of polysaccharides.
Calibration Curves for KS-800 Series (Aqueous Eluent: Pullulan)
Sample: Pullulan
Columns: Shodex SUGAR KS-800 (8.0 x 300 mm each)
Eluent: H2O
Flow rate: 1.0 mL/min
Detector: Shodex RI
Column temp.: 80°C
Calibration curve
SUGAR KS-800 series, a sugar analysis column, is suitable for the analysis of polysaccharides. Calibration curves for the combination of KS-800 series columns of different exclusion limit are shown.
Calibration Curves for KS-800 Series (Column Combination)
Sample: Pullulan
Columns: Shodex SUGAR KS-802, 803, 804 (8.0 x 300 mm each)
Eluent: H2O
Detector: Shodex RI
Column temp.: 80°C
Alternatives
Besides SUGAR KS-800 series, OHpak SB-800 HQ series and Asahipak GS-HQ series also can be used for the analysis of polysaccharides.
For a better separation of monosaccharides, the SUGAR SP0810 (with Pb2+) or SUGAR SC1011 (with Ca2+) is recommended.
Instead of Ligand Exchange chromatography, sugars can also be separated with HILIC separation technique.
TESTIMONIALS
Right product for the right purposes, research made easy
Review date: 18 Jul 2017 | SUGAR KS-801
Application Area: Sugar and sugarcomponents
"The column is easy to use, highly robust and allows direct injections of samples without much pre-treatment"
Olaf Nehlich / imprint analytics on www.selectscience.net