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SUGAR KS-800 columns

with Sulfo (Na+) groups

The Ligand Exchange column for mono-, oligo- and poly-saccharides

Product information

SUGAR KS-801 and KS-802

  • Separates mono- and oligo-saccharides by combination of ligand exchange (Na+) and size exclusion mode

  • Only water is required for the analysis of neutral sugars

KS-803 to 804

  • Suitable for separation of polysaccharides by size exclusion mode

  • Can be used in combination with other columns e.g., KS-802 and KS-801

  • Only water is required for the analysis of neutral sugars

  • Fulfill USP L22 and L58 requirements

These columns are mainly used in routine quality controls in food analysis.

This column series has similar properties like the following references:

BioRad Aminex HPX-87N (Na+), Agilent Hi-Plex PL Na, Phenomenex Rezex RNM-Carbohydrate Na+, Sepax Carbomix Na, Thermo Fisher HyperREZ XP Carbyhydrate Na+, Transgenomic Carbo Sep Coregel 87N, CHO-611.

RSpak DC-613

  • Separates mono- and oligo-saccharides by combination of ligand exchange (Na+) and HILIC mode

  • Water and acetonitrile is used for the analysis

  • DC-613 can analyze sugars without removing sodium salts in the sample

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Size (ID x length)
Housing material
F6378010
SUGAR KS-801
Ligand Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 17,000
6 µm
8.0 x 300 mm
steel
F6378020
SUGAR KS-802
Ligand Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 17,000
6 µm
8.0 x 300 mm
steel
F6378025
SUGAR KS-803
Ligand Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 17,000
6 µm
8.0 x 300 mm
steel
F6378035
SUGAR KS-804
Ligand Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 17,000
7 µm
8.0 x 300 mm
steel
F6700020
SUGAR KS-G 6B
Ligand Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
(guard)
10 µm
6.0 x 50 mm
steel
F7001003
RSpak DC-613
Ligand Exchange + HILIC
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 5,500
6 µm
6.0 x 150 mm
steel
F6700170
RSpak DC-G 4A
Ligand Exchange + HILIC
Sulfo (Na+)
Styrene divinylbenzene copolymer
(guard)
10 µm
4.6 x 10 mm
steel

Product details

Product code
Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
Shipping solvent
USP
Exclusion limit
F6378010
SUGAR KS-801
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.5 mL/min
20 to 85°C
H2O
L22, L58
1,000
F6378020
SUGAR KS-802
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.5 mL/min
20 to 85°C
H2O
L22, L58
10,000
F6378025
SUGAR KS-803
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.5 mL/min
20 to 85°C
H2O
L22, L58
50,000
F6378035
SUGAR KS-804
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.5 mL/min
20 to 85°C
H2O
L22, L58
400,000
F6700020
SUGAR KS-G 6B
-
-
-
20 to 85°C
H2O
L22, L58
-
F7001003
RSpak DC-613
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
3.0 mL/min
40 to 85°C
H2O/ACN = 30/70
L22, L58
-
F6700170
RSpak DC-G 4A
-
-
-
40 to 85°C
H2O/ACN = 30/70
L22, L58
-

Product info

Applications

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SUGAR KS-800

General

Calibration Curves for KS-800 Series (Aqueous Eluent: Pullulan)

Calibration Curves for KS-800 Series (Column Combination)

Explanation on Ligand Exchange Mode

Guidelines for Shodex Column Selection : by Combination of Saccharides

Guidelines for Shodex Column Selection : by Elution Volume

Guidelines for Shodex Column Selection : by Sample Category

Combination of Columns with Different Counter Ions

Comparison of Columns with Different Counter Ions

Elution Volume of Saccharides

Separation of Anomer

Prevention of Anomer Separation

Applications

Amylose (KS-802)

Analysis of Cyclodextrins According to Japan's Specifications and Standards for Food Additives Method (KS-802)

Analysis of Fructooligosaccharide Components (KS-802 + KS-801)

Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)

Analysis of Hydrogenated Maltose Starch Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)

Analysis of Inositol According to Japanese Pharmaceutical Excipients 2018 (KS-801)

Analysis of Maltose According to USP-NF Method (KS-801)

Analysis of Maltose Hydrate According to JP Method (KS-801)

Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)

Analysis of Ribose According to USP-NF Method (KS-801)

Analysis of Saccharides and Furfurals (KS-801)

Analysis of Trehalose According to JP Method (KS-801)

Analysis of Trehalose According to USP-NF Method (KS-801)

Cello-oligosaccharides and Ethanol (KS-802)

Chondroitin Sulfate (KS-804 + KS-801)

Chondroitin Sulfate A (KS-804)

Cyclodextrins (GS-220, KS-802)

Galactooligosaccharides (2) (KS-801, NH2P-50 4E)

Heptulose Separation (KS-801)

Hydrogen Peroxide (KS-801)

Hydrolyzed Dextran (2) (KS-802)

Hydrolyzed Dextran (5) (KS-802)

Hydrolyzed Starch (1)

Hydrolyzed Starch (2)

Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)

Oligosaccharide in Soy Bean (KS-801, KS-802)

Pullulan, Saccharides and Ethanol (KS-802)

Pullulan and Oligosaccharides (KS-804)

Ribavirin (KS-801)

Saccharides and Amino Acids (3) (KS-801)

Saccharides in Bread (KS-801)

Separation of Kestose and Maltose (KS-802)

Starch Syrup (KS-802)

Sugar Alcohols (3) (KS-801)

Sweetener (KS-802)

Worcester Sauce (KS-801)

Xylooligosaccharides (1) (KS-801)

RSpak DC-613

Saccharides Analysis in Ionic Liquid (DC-613)

Saccharides Separation in Presence of Na Salt (DC-613)

Calibration curve

 

SUGAR KS-800 series is suitable to the analysis of polysaccharides. Calibration curves for KS-800 series using pullulan (polysaccahrid standard) are shown here. Besides KS-800 series, OHpak SB-800 HQ series and Asahipak GS-HQ series also can be used for the analysis of polysaccharides.
 

Calibration Curves for KS-800 Series (Aqueous Eluent: Pullulan)
Sample: Pullulan

Columns: Shodex SUGAR KS-800 (8.0 x 300 mm each)

Eluent: H2O

Flow rate: 1.0 mL/min

Detector: Shodex RI

Column temp.: 80°C

s1002c.gif

Calibration curve

 

SUGAR KS-800 series, a sugar analysis column, is suitable for the analysis of polysaccharides. Calibration curves for the combination of KS-800 series columns of different exclusion limit are shown.

Calibration Curves for KS-800 Series (Column Combination)
Sample: Pullulan

Columns: Shodex SUGAR KS-802, 803, 804 (8.0 x 300 mm each)

Eluent: H2O

Detector: Shodex RI

Column temp.: 80°C

s1003.gif

Alternatives

Besides SUGAR KS-800 series, OHpak SB-800 HQ series and Asahipak GS-HQ series also can be used for the analysis of polysaccharides.
 

For a better separation of monosaccharides, the SUGAR SP0810 (with Pb2+) or SUGAR SC1011 (with Ca2+) is recommended.

Instead of Ligand Exchange chromatography, sugars can also be separated with HILIC separation technique.

TESTIMONIALS

Right product for the right purposes, research made easy

Review date: 18 Jul 2017 | SUGAR KS-801

 

Application Area: Sugar and sugarcomponents

"The column is easy to use, highly robust and allows direct injections of samples without much pre-treatment"
Olaf Nehlich / imprint analytics on www.selectscience.net

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