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Shodex OHpak SB Series_43.jpg

OHpak SB-800  columns

separation by size for a variety of molecular weights

The polymer-based SEC (GFC) column for modified proteins, PEGs, polysaccharides, surfactants

Product information

OHpak SB-800 HQ series

  • Polymer-based packed columns for aqueous SEC (GFC) analysis

  • Supports a wide range of molecular weight sample analysis

  • The eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ), enabling the analysis of polar polymers

  • Fomodified proteins, PEGs, polysaccharides (hyaluronic acid, alginates, ...), surfactants

OHpak SB-807 HQ

  • Column for the analysis of water-soluble ultra high molecular weight polymers

  • Large particle-size gel prevents shear degradation of polymers

OHpak SB-2000 series

  • Preparative size with 20.0 x 300 mm

For Light Scattering detectors: special series with higher crosslinking with ultra-low bleeding: OHpak LB-800 series

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F6429100
OHpak SB-802 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
8 µm
100 Å
8.0 x 300 mm
steel
F6429101
OHpak SB-802.5 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 16,000
6 µm
200 Å
8.0 x 300 mm
steel
F6429102
OHpak SB-803 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 16,000
6 µm
800 Å
8.0 x 300 mm
steel
F6429103
OHpak SB-804 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 16,000
10 µm
2,000 Å
8.0 x 300 mm
steel
F6429104
OHpak SB-805 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
13 µm
7,000 Å
8.0 x 300 mm
steel
F6429105
OHpak SB-806 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
13 µm
15,000 Å
8.0 x 300 mm
steel
F6429106
OHpak SB-806M HQ
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
13 µm
15,000 Å
8.0 x 300 mm
steel
F6709430
OHpak SB-G 6B
SEC
-
Polyhydroxy-methacrylate
(guard)
10 µm
-
6.0 x 50 mm
steel
F6429108
OHpak SB-807 HQ
SEC
-
Polyhydroxy-methacrylate
≥ 1,500
35 µm
30,000 Å
8.0 x 300 mm
steel
F6709431
OHpak SB-807G
SEC
-
Polyhydroxy-methacrylate
(guard)
35 µm
-
8.0 x 50 mm
steel
F6516011
OHpak SB-2002
SEC
-
Polyhydroxy-methacrylate
≥ 9,000
15 µm
100 Å
20.0 x 300 mm
steel
F6516012
OHpak SB-2002.5
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
10 µm
200 Å
20.0 x 300 mm
steel
F6516013
OHpak SB-2003
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
10 µm
800 Å
20.0 x 300 mm
steel
F6516014
OHpak SB-2004
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
18 µm
2,000 Å
20.0 x 300 mm
steel
F6516015
OHpak SB-2005
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
20 µm
7,000 Å
20.0 x 300 mm
steel
F6516016
OHpak SB-2006
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
20 µm
15,000 Å
20.0 x 300 mm
steel
F6516017
OHpak SB-2006M
SEC
-
Polyhydroxy-methacrylate
≥ 12,000
20 µm
15,000 Å
20.0 x 300 mm
steel
F6709555
OHpak SB-G 8B
SEC
-
Polyhydroxy-methacrylate
(guard)
18 µm
-
8.0 x 50 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
MW range
Exclusion limit
OHpak SB-802 HQ
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L25, L38, L39
200 - 1,000
1,000
OHpak SB-802.5 HQ
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L25, L38, L39, L89
500 - 10,000
10,000
OHpak SB-803 HQ
5.0 MPa (50 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L37, L38, L39
1,000 - 100,000
100,000
OHpak SB-804 HQ
3.0 MPa (30 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
5,000 - 400,000
1,000,000
OHpak SB-805 HQ
3.0 MPa (30 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
100,000 - 1,000,000
(4,000,000)
OHpak SB-806 HQ
3.0 MPa (30 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
100,000 - (20,000,000)
(20,000,000)
OHpak SB-806M HQ
3.0 MPa (30 bar)
0.5 to 1.0 mL/min
1.2 mL/min
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
500 - (20,000,000)
(20,000,000)
OHpak SB-G 6B
-
-
-
4 to 70°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
-
-
OHpak SB-807 HQ
0.5 MPa (5 bar)
0.5 to 1.0 mL/min
1.5 mL/min
4 to 60°C
3 to 10
≤ 0.5 M
H2O
L38, L39
500,000 - (500,000,000)
(500,000,000)
OHpak SB-807G
-
-
-
4 to 60°C
3 to 10
≤ 0.5 M
H2O
L38, L39
-
-
OHpak SB-2002
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L25, L38, L39
200 - 1,000
1,000
OHpak SB-2002.5
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L25, L38, L39
500 - 10,000
10,000
OHpak SB-2003
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
1,000 - 100,000
100,000
OHpak SB-2004
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
5,000 - 400,000
1,000,000
OHpak SB-2005
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
100,000 - 1,000,000
(4,000,000)
OHpak SB-2006
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
100,000 - (20,000,000)
(20,000,000)
OHpak SB-2006M
2.0 MPa (20 bar)
3.0 mL/min
5.0 mL/min
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
500 - (20,000,000)
(20,000,000)
OHpak SB-G 8B
-
-
-
15 to 60°C
3 to 10
≤ 0.5 M
0.02 % NaN3 aq.
L38, L39
-
-

Products

Applications

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General OHpak SB-800

Calibration Curves for SB-800 HQ Series (Aqueous Eluent: PEG, PEO)

Calibration Curves for SB-800 HQ Series (Aqueous Eluent: Pullulan)

Calibration Curves for SB-800 HQ Series (DMF Eluent: PEG, PEO)

Calibration Curves for SB-800 HQ Series (DMF Eluent: PMMA)

Calibration Curves for SB-800 HQ Series (DMF Eluent: PS)

Calibration Curves for SB-806M HQ (Aqueous Eluent: Pullulan, PEG and PEO)

Calibration Curves for SB-806M HQ (DMF Eluent: PEG, PEO and PS)

Calibration Curve for SB-807 HQ (Aqueous Eluent: Pullulan)

Comparison of Calibration Curves for KD-800 Series and SB-800 HQ Series (DMF Eluent)

Comparison of Calibration Curves for KD-802.5 and SB-802.5 HQ (DMF Eluent: PEG, PEO and PS)

Comparison of Chromatogram of PEG Mixtures

Comparison of Chromatograms of Protein mixtures

Effects of Sample Load (Pullulan)

Hydrophobicity of Columns

Analysis OHpak SB-800 HQ

Human Serum (1) (SB-802 HQ)

Adrenal Cortical Hormones (1) (SB-802.5 HQ)

Amino Sugars (SB-802.5 HQ)

Analysis of Ferric Carboxymaltose (SB-804 HQ + SB-802.5 HQ)

Analysis of Purified Sodium Hyaluronate Ophthalmic Solution According to JP Method (SB-802.5 HQ)

Analysis of Urea, an Impurity in Fluorouracil Injection, Proposed in USP-NF Pharmacopeial Forum (SB-802.5 HQ)

Assay of Polyethylene Glycol 3350 According to USP-NF Method (SB-802.5 HQ)

Assay of Polyethylene Glycol 3350 for Oral Solution According to USP-NF Method (SB-802.5 HQ)

Benzalkonium Chloride (3) (SB-802.5 HQ)

Ethylene Maleic Anhydride Copolymer (Comparison of KD-800 and SB-800 HQ)

Hydroxypropyl-β-cyclodextrin (SB-802.5 HQ)

Phenol Formaldehyde Resin (Comparison of KD-800 and SB-800 HQ)

Poly(-N,N-Dimethylaminomethyl Styrene) (1) (DMF Eluent) (Comparison KD-800 with SB-800 HQ)

Poly(Ethylene Glycol) Standards (8) (SB-802.5 HQ)

Polycarbonate Resin (2) (Comparison of SB-800 HQ and KD-800)

Polyphosphates (2) (SB-802.5 HQ)

SEC Analysis of Saccharated Ferric Oxide Injection (SB-804 HQ + SB-802.5 HQ)

Simultaneous Analysis of Anionic and Non-ionic Surfactants

Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)

Steroides

Trimethylbenzylammonium Chloride (SB-802.5 HQ)

Triton X-100 (SB-802.5 HQ)

Vinylidene Chloride Acrylonitrile Copolymer (2) (Comparison of KD-800 and SB-800 HQ)

Vitamin B12 (SB-802.5 HQ)

Analysis of Betadex Sulfobutyl Ether Sodium According to USP-NF Method (SB-803 HQ)

Analysis of Ferric Carboxymaltose (SB-804 HQ + SB-802.5 HQ)

Analysis of Humic Substances by SEC (SB-805 HQ)

Bibliographic Information for Analysis of Conjugate Vaccine

Chitosan (SB-806M HQ)

Colominic Acid (SB-804 HQ)

Dextran (1) (SB-805 HQ)

Ethylene Maleic Anhydride Copolymer (Comparison of KD-800 and SB-800 HQ)

Heparin (1) (SB-804 HQ)

Human IgM (2)

Hyaluronic Acid (1) (SB-805 HQ)

Measurement of Apparent Weight-Average Molecular Weight and Polydispersity of Polyethylene Glycol 3350 According to USP-NF Method (SB-803 HQ)

Phenol Formaldehyde Resin (Comparison of KD-800 and SB-800 HQ)

Plasma Lipoproteins (1) (SB-805 HQ)

Poly(-N,N-Dimethylaminomethyl Styrene) (1) (DMF Eluent) (Comparison KD-800 with SB-800 HQ)

Poly(Ethylene Imine) (SB-804 HQ + SB-803 HQ)

Poly(Ethylene Oxide) and Poly(Ethylene Glycol) Standards (SB-804 HQ)

Poly(Vinyl Alcohol) (2) (Aqueous Eluent) (SB-806 HQ + SB-803 HQ)

Polyacrylamide (1) (SB-805 HQ)

Polycarbonate Resin (2) (Comparison of SB-800 HQ and KD-800)

Polyvinylpyrrolidone (3) (NaCl aq./CH3CN Eluent) (SB-806M HQ)

Pullulan Standards (1) (SB-804 HQ)

SEC Analysis of Saccharated Ferric Oxide Injection (SB-804 HQ + SB-802.5 HQ)

SEC/MALS Analysis of Norovirus VLP (SB-805 HQ)

Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)

Vinylidene Chloride Acrylonitrile Copolymer (2) (Comparison of KD-800 and SB-800 HQ)

Analysis of Gelatin (SB-806M HQ)

Analysis of Cyanobacterial Glycogen Using SEC Column (SB-806M HQ)

Analysis of Proteoglycan in a Supplement (SB-806M HQ)

Arabic Gum (SB-806M HQ)

Bibliographic Information for Analysis of Conjugate Vaccine

Carboxymethylcellulose (3) (SB-806M HQ)

Carboxymethylcellulose (4) (SB-806M HQ)

Carrageenan (SB-806M HQ)

Cellulose Acetate (5) (SB-806M HQ)

Characterization of Polymeric Excipients by SEC-IR (SB-806M HQ)

Chitosan (SB-806M HQ)

Chondroitin Sulfate (SB-806M HQ)

Copovidone (SB-806M HQ)

Corn Starch (1) (Aqueous Eluent) (SB-806M HQ)

Corn Starch (2) (DMSO/DMF Eluent) (SB-806 HQ)

Dextran (3) (SB-806M HQ)

Fucoidan (SB-806M HQ)

Glycogen (2) (SB-806M HQ)

Guar Gum (SB-806M HQ)

Heparin (2) (SB-806M HQ)

Hyaluronic Acid (2) (SB-806M HQ)

Hydroxyethyl Cellulose (3) (SB-806M HQ)

Hydroxyethyl Cellulose (4) (SB-806M HQ)

Hydroxypropyl Cellulose (SB-806M HQ)

Hydroxypropyl Methylcellulose (SB-806M HQ)

Inulin (2) (SB-806M HQ)

Keratan sulfate (SB-806M HQ)

Laminarin (SB-806M HQ)

Measurement of Molecular Weight Distribution of Poly(Allylamine) Hydrochloride (SB-806M HQ)

Measurement of Molecular Weight Distribution of Poly(Diallyl Dimethyl Ammonium Chloride) (SB-806M HQ)

Measurement of Molecular Weight Distribution of Poly(Ethylene Imine) (SB-806M HQ)

Methylcellulose (SB-806M HQ)

Mucin (SB-806M HQ)

Pectin (SB-806M HQ)

Poly(Vinyl Alcohol) (2) (Aqueous Eluent) (SB-806 HQ + SB-803 HQ)

Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)

Polyadenylic Acid (SB-806 HQ)

Polyvinylpyrrolidone (6) (NaCl aq./CH3CN Eluent) (SB-806M HQ)

SEC Analysis of PEG-Asparaginase (SB-806 HQ)

SEC/MALS Analysis of Exosome (SB-806 HQ)

Selection of Column (1) (Sodium Poly(Styrene Sulfonate))

Selection of Column (2) (Sodium Polyacrylate)

Selection of Column (3) (Poly(Sodium Methacrylate))

Sodium Alginate (SB-806M HQ)

Sodium Dextran Sulfate (SB-806M HQ)

Hyaluronic Acid (4) (SB-807 HQ)

Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)

Preparative OHpak SB-2000

Poly(Ethylene Glycol) Standards (7) (SB-2002.5)

Pullulan Standards (4) (Effects of Flow Rate) (SB-2002.5)

Pullulan Standards (5) (Effects of Temperature) (SB-2002.5)

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