Shodex Asahipak ODP-50 Column Series

Asahipak ODP-50 columns

with C18 groups

The robust polymer-based RP column for many general applications

Download the product overview (pdf)

Product information

 

Asahipak ODP-50

The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis.

  • Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins

  • Usable in a wide pH range from pH 2 to 13

  • Usable in 100 % water and buffer solution

  • Best used for the analysis of basic substances

  • Fulfills USP L67 requirements

  • Also available as Asahipak C4P-50 with C4 groups

  • Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)

Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials.

 

Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns:
A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions).

Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides. The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant.


Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs.

RP comparison.jpg

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F7620009
Asahipak ODP-50 2D
RP
C18
Polyvinyl alcohol
≥ 5,000
5 µm
250 Å
2.0 x 150 mm
steel
F6713001
Asahipak ODP-50G 2A
RP
C18
Polyvinyl alcohol
(guard)
5 µm
-
2.0 x 10 mm
steel
F6710023
Asahipak ODP-50 4B
RP
C18
Polyvinyl alcohol
≥ 2,500
5 µm
250 Å
4.6 x 50 mm
steel
F7620004
Asahipak ODP-50 4D
RP
C18
Polyvinyl alcohol
≥ 9,000
5 µm
250 Å
4.6 x 150 mm
steel
F7620003
Asahipak ODP-50 4E
RP
C18
Polyvinyl alcohol
≥ 14,000
5 µm
250 Å
4.6 x 250 mm
steel
F6710022
Asahipak ODP-50G 4A
RP
C18
Polyvinyl alcohol
(guard)
5 µm
-
4.6 x 10 mm
steel
F7620002
Asahipak ODP-50 6D
RP
C18
Polyvinyl alcohol
≥ 9,000
5 µm
250 Å
6.0 x 150 mm
steel
F7620001
Asahipak ODP-50 6E
RP
C18
Polyvinyl alcohol
≥ 14,000
5 µm
250 Å
6.0 x 250 mm
steel
F6710001
Asahipak ODP-50G 6A
RP
C18
Polyvinyl alcohol
(guard)
5 µm
-
6.0 x 10 mm
steel
F7620008
Asahipak C4P-50 4D
RP
C4
Polyvinyl alcohol
≥ 6,000
5 µm
250 Å
4.6 x 150 mm
steel
F6710003
Asahipak C4P-50G 4A
RP
C4
Polyvinyl alcohol
(guard)
5 µm
-
4.6 x 10 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
Info
Asahipak ODP-50 2D
12 MPa (120 bar)
0.2 mL/min
0.4 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50G 2A
-
-
-
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50 4B
5 MPa (50 bar)
0.4 to 0.6 mL/min
1.5 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50 4D
15 MPa (150 bar)
0.5 to 0.8 mL/min
1.5 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50 4E
15 MPa (150 bar)
0.5 to 0.8 mL/min
1.5 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50G 4A
-
-
-
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50 6D
15 MPa (150 bar)
0.5 to 1.0 mL/min
2.5 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50 6E
15 MPa (150 bar)
0.5 to 1.0 mL/min
2.5 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak ODP-50G 6A
-
-
-
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
L67
Carbon load 17%
Asahipak C4P-50 4D
15 MPa (150 bar)
0.5 to 0.8 mL/min
1.0 mL/min
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
-
Carbon load 6%
Asahipak C4P-50G 4A
-
-
-
4 to 60°C
2 to 13
≤ 0.1 M
H2O/ACN = 35/65
-
Carbon load 6%

Product info