The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis.
Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins
Usable in a wide pH range from pH 2 to 13
Usable in 100 % water and buffer solution
Best used for the analysis of basic substances
Fulfills USP L67 requirements
Also available as Asahipak C4P-50 with C4 groups
Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)
Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials.
Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns:
A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions).
Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides.
The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant.
Shodex is specialized in p