Anion exchange columns

strong and weak anion exchange

Suitable for biomolecules like proteins, peptides, DNA, RNA

Product information

Ion exchange chromatography is an analytical method relying on the interaction of ion-charges between the surface of proteins and the surface of the packing material. Packing materials with a positive surface charge are called “anion exchangers” and those with a negative surface charge are called “cation exchangers”.

A strong anion exchange resin with a quaternary ammonium functional group is packed into the IEC QA-825 column. This columncan be used at a wider pH range because the pKa of the ion exchange base is 11.7.

IEC QA-825

Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12
QA-825 fulfills USP L23 requirements

A weak anion exchange resin with a diethylaminoethyl (DEAE) functional group is packed into IEC DEAE-825 and Asahipak ES-502N columns. These columns are suitable for the separation of acidic proteins because the pKa of the ion exchanger is about 7.8.

IEC DEAE-825

Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12

Asahipak ES-502N 7C

Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12
Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern
Low hydrophobic interaction of proteins allows analysis under mild conditions

Obtaining some information about the isoelectric point (pI) of proteins is useful for selecting suitable analytical conditions. Proteins consist of amino acids, and are amphoteric compounds, so there is pH at which the total sum of electrical charges becomes zero in the solution. That pH is called isoelectric point (pI). Around the pI, the electrical charge on the surface of proteins is neutralized and such proteins cannot easily adsorb on the surface of the packing material. In some cases, proteins precipitate around the pI, a phenomenon known as “isoelectric precipitation”. In order to adsorb the target protein, the pH of the eluent must be adjusted at more than 1.0 pH unit from pI. When the pH is more acidic than the pI, a cation exchanger should be used, and when the pH is more alkaline than the pI, an anion exchanger should be selected.

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F6110011	IEC QA-825	Ion Exchange	Quaternary ammonium	Polyhydroxy-methacrylate	≥ 2,000	12 µm	5,000 Å	8.0 x 75 mm	steel
F6118255	IEC DEAE-825	Ion Exchange	Diethylaminoethyl	Polyhydroxy-methacrylate	≥ 2,000	8 µm	5,000 Å	8.0 x 75 mm	steel
F7640002	Asahipak ES-502N 7C	Ion Exchange	Diethylaminoethyl	Polyvinyl alcohol	≥ 3,300	9 µm	2,000 Å	7.5 x 100 mm	steel

Product details

Product name	Maximum pressure	Usual flow rate	Maximum flow rate	Temperature range	pH range	Salt concentration	Shipping solvent	USP	Info
IEC QA-825	2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 1.0 M	50 mM Na2SO4 aq.	L23	0.45 meq/g Ion-exchange capacity
IEC DEAE-825	2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 1.0 M	50 mM Na2SO4 aq.	-	0.6 meq/g Ion-exchange capacity
Asahipak ES-502N 7C	1.2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 0.6 M	50 mM 1,3-Diaminopropane + 50 mM NaCl (pH 10.0)	-	0.55 meq/g Ion-exchange capacity

Products

Download documents

Operation Manual IEC DEAE-825_20221202E

Operation Manual IEC QA-825_20221202E

Operation Manual Asahipak ES-502N 7C_20221202E

Bedienungsanleitung IEC DEAE-825_20221202G

Bedienungsanleitung IEC QA-825_20221202G

Bedienungsanleitung Asahipak ES-502N 7C_20221202G

Applications

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IEC QA-825

Titration Curve of IEC QA-825

Eluent Conditions for IEC-series

Bovine Liver Catalase

Crude Albumin (Chicken Egg)

Effects of Sample Load on Resolution (QA-825)

Lipoxidase from Soybean (1) (QA-825, DEAE-825)

Proteins in Human Serum (1) (QA-825)

Purified Kallikrein from Pig Pancreas

Recovery of Proteins and Enzime Activity (QA-825)

Standard Proteins (5) (QA-825)

IEC DEAE-825