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Cation exchange columns

strong and weak cation exchange

Suitable for biomolecules like proteins, peptides, DNA, RNA

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Product information

Ion exchange chromatography is an analytical method relying on the interaction of ion-charges between the surface of proteins and the surface of the packing material. Packing materials with a positive surface charge are called “anion exchangers” and those with a negative surface charge are called “cation exchangers”.

A strong cation exchange resin with a sulfopropyl functional group is packed into the IEC SP-825 and IEC SP-FT 4A column. This columns can be used at a wider pH range because the pKa of the ion exchange base is 2.3.

IEC SP-825

  • Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA

  • Usable in a wide pH range from pH 2 to 12

 

IEC SP-FT 4A

  • Non-porous base material

  • Provides ultra-rapid analysis using conventional devices

  • PEEK housing

IEC CM-825 and Asahipak ES-502C are packed with a weak cation exchange resin with Carboxymethyl as the functional group. These
columns are suitable for the separation of basic proteins because the pKa of the ion exchange base is 5.7.

IEC CM-825

  • Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA

  •  Usable in a wide pH range from pH 2 to 12

 

Asahipak ES-502C 7C

  • Compared to IEC series columns, polyvinyl alcohol is used as base material offering different separation pattern

  • Low hydrophobic interaction with proteins allows analysis under mild conditions

 

CXpak P-421 is a strong cation exchange column filled with spherical porous particles of styrene divinylbenzene copolymer. It is an ideal column for analyzing various amino acids. CXpak P-421 is used with an amino acid analyzer with post-column derivatization method.

CXpak P-421S

  • Column for amino acids analysis by cation exchange mode

  • Provides simultaneous analysis of different amino acids

  • Fulfills USP L22 and L58 requirements


Obtaining some information about the isoelectric point (pI) of proteins is useful for selecting suitable analytical conditions. Proteins consist of amino acids, and are amphoteric compounds, so there is pH at which the total sum of electrical charges becomes zero in the solution. That pH is called isoelectric point (pI). Around the pI, the electrical charge on the surface of proteins is neutralized and such proteins cannot easily adsorb on the surface of the packing material. In some cases, proteins precipitate around the pI, a phenomenon known as “isoelectric precipitation”. In order to adsorb the target protein, the pH of the eluent must be adjusted at more than 1.0 pH unit from pI. When the pH is more acidic than the pI, a cation exchanger should be used, and when the pH is more alkaline than the pI, an anion exchanger should be selected.

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F6118250
IEC SP-825
Ion Exchange
Sulfopropyl
Polyhydroxy-methacrylate
≥ 2,000
8 µm
5,000 Å
8.0 x 75 mm
steel
F6113100
IEC SP-FT 4A
Ion Exchange
Sulfopropyl
Polyhydroxy-methacrylate
≥ 20,000
2.7 µm
-
4.6 x 10 mm
PEEK
F6110002
IEC CM-825
Ion Exchange
Carboxymethyl
Polyhydroxy-methacrylate
≥ 2,000
8 µm
5,000 Å
8.0 x 75 mm
steel
F7640001
Asahipak ES-502C 7C
Ion Exchange
Carboxymethyl
Polyvinyl alcohol
≥ 3,300
9 µm
2,000 Å
7.5 x 100 mm
steel
F6354211
CXpak P-421S
Ion Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
≥ 3,500
6 µm
-
4.6 x 150 mm
steel
F6700210
CXpak P-G
Ion Exchange
Sulfo (Na+)
Styrene divinylbenzene copolymer
(guard)
6 µm
-
4.6 x 10 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
Info
IEC SP-825
2 MPa (20 bar)
1.0 mL/min
1.5 mL/min
10 to 50°C
2 to 12
20 mM to 1.0 M
50 mM Na2SO4 aq.
-
0.4 meq/g Ion-exchange capacity
IEC SP-FT 4A
20 MPa (200 bar)
1.0 mL/min
3.0 mL/min
5 to 45°C
2 to 12
≤ 1.5 M
20 mM 2-(N-Morpholino)ethanesulfonic acid buffer (pH 5.6)
-
0.2 meq/g Ion-exchange capacity
IEC CM-825
2 MPa (20 bar)
1.0 mL/min
1.5 mL/min
10 to 50°C
2 to 12
20 mM to 1.0 M
50 mM Na2SO4 aq.
-
0.4 meq/g Ion-exchange capacity
Asahipak ES-502C 7C
1.2 MPa (20 bar)
1.0 mL/min
1.5 mL/min
10 to 50°C
2 to 12
20 mM to 0.6 M
0.1 M Sodium phosphate buffer (pH 4.4)
-
0.55 meq/g Ion-exchange capacity
CXpak P-421S
5 MPa (50 bar)
0.5 mL/min
0.7 mL/min
15 to 63°C
≥ 3
-
H2O
L22, L58
-
CXpak P-G
-
-
-
15 to 63°C
≥ 3
-
H2O
L22, L58
-

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