Cation exchange columns

strong and weak cation exchange

Suitable for biomolecules like proteins, peptides, DNA, RNA

Product information

Ion exchange chromatography is an analytical method relying on the interaction of ion-charges between the surface of proteins and the surface of the packing material. Packing materials with a positive surface charge are called “anion exchangers” and those with a negative surface charge are called “cation exchangers”.

A strong cation exchange resin with a sulfopropyl functional group is packed into the IEC SP-825 and IEC SP-FT 4A column. This columns can be used at a wider pH range because the pKa of the ion exchange base is 2.3.

IEC SP-825

Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12

IEC SP-FT 4A

Non-porous base material
Provides ultra-rapid analysis using conventional devices
PEEK housing

IEC CM-825 and Asahipak ES-502C are packed with a weak cation exchange resin with Carboxymethyl as the functional group. These
columns are suitable for the separation of basic proteins because the pKa of the ion exchange base is 5.7.

IEC CM-825

Suitable for analyzing relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
Usable in a wide pH range from pH 2 to 12

Asahipak ES-502C 7C

Compared to IEC series columns, polyvinyl alcohol is used as base material offering different separation pattern
Low hydrophobic interaction with proteins allows analysis under mild conditions

CXpak P-421 is a strong cation exchange column filled with spherical porous particles of styrene divinylbenzene copolymer. It is an ideal column for analyzing various amino acids. CXpak P-421 is used with an amino acid analyzer with post-column derivatization method.

CXpak P-421S

Column for amino acids analysis by cation exchange mode
Provides simultaneous analysis of different amino acids
Fulfills USP L22 and L58 requirements

Obtaining some information about the isoelectric point (pI) of proteins is useful for selecting suitable analytical conditions. Proteins consist of amino acids, and are amphoteric compounds, so there is pH at which the total sum of electrical charges becomes zero in the solution. That pH is called isoelectric point (pI). Around the pI, the electrical charge on the surface of proteins is neutralized and such proteins cannot easily adsorb on the surface of the packing material. In some cases, proteins precipitate around the pI, a phenomenon known as “isoelectric precipitation”. In order to adsorb the target protein, the pH of the eluent must be adjusted at more than 1.0 pH unit from pI. When the pH is more acidic than the pI, a cation exchanger should be used, and when the pH is more alkaline than the pI, an anion exchanger should be selected.

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F6118250	IEC SP-825	Ion Exchange	Sulfopropyl	Polyhydroxy-methacrylate	≥ 2,000	8 µm	5,000 Å	8.0 x 75 mm	steel
F6113100	IEC SP-FT 4A	Ion Exchange	Sulfopropyl	Polyhydroxy-methacrylate	≥ 20,000	2.7 µm	-	4.6 x 10 mm	PEEK
F6110002	IEC CM-825	Ion Exchange	Carboxymethyl	Polyhydroxy-methacrylate	≥ 2,000	8 µm	5,000 Å	8.0 x 75 mm	steel
F7640001	Asahipak ES-502C 7C	Ion Exchange	Carboxymethyl	Polyvinyl alcohol	≥ 3,300	9 µm	2,000 Å	7.5 x 100 mm	steel
F6354211	CXpak P-421S	Ion Exchange	Sulfo (Na+)	Styrene divinylbenzene copolymer	≥ 3,500	6 µm	-	4.6 x 150 mm	steel
F6700210	CXpak P-G	Ion Exchange	Sulfo (Na+)	Styrene divinylbenzene copolymer	(guard)	6 µm	-	4.6 x 10 mm	steel

Product details

Product name	Maximum pressure	Usual flow rate	Maximum flow rate	Temperature range	pH range	Salt concentration	Shipping solvent	USP	Info
IEC SP-825	2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 1.0 M	50 mM Na2SO4 aq.	-	0.4 meq/g Ion-exchange capacity
IEC SP-FT 4A	20 MPa (200 bar)	1.0 mL/min	3.0 mL/min	5 to 45°C	2 to 12	≤ 1.5 M	20 mM 2-(N-Morpholino)ethanesulfonic acid buffer (pH 5.6)	-	0.2 meq/g Ion-exchange capacity
IEC CM-825	2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 1.0 M	50 mM Na2SO4 aq.	-	0.4 meq/g Ion-exchange capacity
Asahipak ES-502C 7C	1.2 MPa (20 bar)	1.0 mL/min	1.5 mL/min	10 to 50°C	2 to 12	20 mM to 0.6 M	0.1 M Sodium phosphate buffer (pH 4.4)	-	0.55 meq/g Ion-exchange capacity
CXpak P-421S	5 MPa (50 bar)	0.5 mL/min	0.7 mL/min	15 to 63°C	≥ 3	-	H2O	L22, L58	-
CXpak P-G	-	-	-	15 to 63°C	≥ 3	-	H2O	L22, L58	-

Products

Download documents

Operation Manual IEC CM-825_20221202E

Operation Manual IEC SP-825_20221202E

Operation Manual IEC SP-FT 4A_20221202E

Operation Manual Asahipak ES-502C 7C_20221202E

Operation Manual CXpak P-421S_20221202E

Bedienungsanleitung IEC CM-825_20221202G

Bedienungsanleitung IEC SP-825_20221202G

Bedienungsanleitung IEC SP-FT 4A_20221202G

Bedienungsanleitung Asahipak ES-502C 7C_20221202G

Bedienungsanleitung CXpak P-421S_20221202G

Applications

At the moment a new window opens and you are transferred to our Japanese website, when you click on the application. We are working on a better solution.

IEC SP-825

Titration Curve of IEC SP-825

Analysis of Thaumatin (SP-825)

Angiotensin Ⅰ, Ⅱ, Ⅲ

Effects of pH on Elution Pattern (SP-825)

Effects of Sample Load on Peak Width (SP-825)

Lipoxidase (1) (SP-825)

Papain

Recovery of Proteins and Enzyme Activity (SP-825)

Standard Proteins (7) (CM-825, SP-825)

IEC SP-FT 4A

Charge Variant Analysis of Cetuximab (SP-FT 4A)