Chiral ORpak CDBS-453 column
with β-Cyclodextrin derivatives
For the separation of Epinephrine and other chiral drugs
Product information
In the fields of medicine, agricultural chemicals and food, it is important to analyze optical isomers to confirm physiological activity or to measure optical purity of optical isomers. Shodex offers one type of separation column of chiral separation.
ORpak CDBS-453
The ORpak CDBS-453 column is packed with sperical porous silica particles bonded with β-cyclodextrin derivatives as ligand.
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Separates optical isomers by using their conformational compatibility differences
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Versatile column for chiral separation
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Fulfills USP L45 requirements
A guard column is not available.
Analytes: Epinephrine, Norepinephrine, Lamivudine, Voriconazole and other chiral drugs
Example:
Sample: 20 μL Epinephrine (racemic mixture) 50 μg/mL (in eluent)
Column: Shodex ORpak CDBS-453 (4.6 x 150 mm)
Eluent: (0.05 % (w/v) CH3COOH + 0.2 M NaCl) aq./CH3CN=95/5
Flow rate: 0.5 mL/min
Detector: UV (280 nm)
Column temp.: 10°C
For some demanding methods, a higher separation efficiency than normal is required; for this, we offer a special selection of a resolution > 2.1 for Noradrenaline (=Norepinephrine). The product code is F7146999, ORpak CDBS-453 SP, with a higher list price of 1.664,00 EUR.
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F7146003 | ORpak CDBS-453 | Chiral | beta-Cyclodextrin derivate | Silica | ≥ 6,000 | 3 µm | - | 4.6 x 150 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP |
|---|---|---|---|---|---|---|---|---|
ORpak CDBS-453 | 20 MPa (200 bar) | 0.3 - 0.8 mL/min | 1.2 mL/min | 5 to 60°C | 2 to 7 | ≤ 1.0 M | (0.05 % CH3COOH + 0.2 M NaCl aq.)/CH3CN/ = 95/5 | L45 |
Download documents
Operation Manuals:
Operation manuals for each column series can be found on our Japanese website.
Please click the following link to be directed to the Operation Manual Database.
Applications
Please take a look at the following list of example application data and chromatograms obtained with our columns. These links will redirect you to our Japanese website.
Analysis of Epinephrine Injection Proposed in USP-NF Pharmacopeial Forum (CDBS-453)
Chiral Separation of Norepinephrine (CDBS-453)
Impurity Analysis of Lamivudine According to USP-NF Method (CDBS-453)
Impurity Analysis of Voriconazole According to USP-NF Method (CDBS-453)