Shodex PROTEIN Series_43.jpg

PROTEIN KW/ PROTEIN LW columns

separation by size also for high molecular weight

The silica-based SEC (GFC) column for proteins and enzymes

Download the product overview (pdf)

Product information

 

PROTEIN KW-800 series
The packing material of the Shodex PROTEIN KW-800 series is based on silica particles whose surface is coated with a hydrophilic polymer. This column is for aqueous SEC (Gel filtration chromatography (GFC)). It enjoys a positive reputation thanks to its high recovery ratio of proteins and peptide. The PROTEIN KW-800 series comprises three kinds of columns, differing in pore and particle size.

  • Silica-based packed columns for aqueous SEC (GFC) analysis

  • Suitable for the analysis of proteins and enzymes

  • Fulfills USP-NF L20, L33, and L59 requirements

PROTEIN KW400 series
The Shodex KW400-4F series is the high-performance semi-micro column version of the KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. KW405-4F, the newest addition to the KW400 line-up, has a larger pore size than any columns in the existing KW-800 series, and can analyze proteins whose molecular weight is larger than 1,000,000. It enables the scientist to carry out the analysis of protein complexes.

  • Reduced packing material particle size enhances column performance

  • Three to four-fold higher sensitivity than KW-800 series

  • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000

  • Fulfills USP-NF L20, L33, and L59 requirements

PROTEIN LW-803 and LW-403 4D

More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.

  • Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand

  • High performance analysis of antibody drugs and various proteins

  • High lot-to-lot reproducibility

  • Fulfills USP-NF L20, L33, and L59 requirements

Product overview

Product code
Product name
Separation
Functional group
Gel material
Plates per column
Particle size
Pore size
Size (ID x length)
Housing material
F6989000
PROTEIN KW-802.5
SEC
Hydroxyl
Silica
≥ 21,000
5 µm
150 Å (max. 400 Å)
8.0 x 300 mm
steel
F6989103
PROTEIN KW-803
SEC
Hydroxyl
Silica
≥ 21,000
5 µm
300 Å (max. 1,000 Å)
8.0 x 300 mm
steel
F6989104
PROTEIN KW-804
SEC
Hydroxyl
Silica
≥ 16,000
7 µm
500 Å (max. 1,500 Å)
8.0 x 300 mm
steel
F6700131
PROTEIN KW-G 6B
SEC
Hydroxyl
Silica
(guard)
7 µm
-
6.0 x 50 mm
steel
F6989201
PROTEIN KW402.5-4F
SEC
Hydroxyl
Silica
≥ 35,000
3 µm
150 Å (max. 400 Å)
4.6 x 300 mm
steel
F6989202
PROTEIN KW403-4F
SEC
Hydroxyl
Silica
≥ 35,000
3 µm
250 Å (max. 800 Å)
4.6 x 300 mm
steel
F6989203
PROTEIN KW404-4F
SEC
Hydroxyl
Silica
≥ 25,000
5 µm
500 Å (max. 1,500 Å)
4.6 x 300 mm
steel
F6989204
PROTEIN KW405-4F
SEC
Hydroxyl
Silica
≥ 25,000
5 µm
1,000 Å (max. 2,000 Å)
4.6 x 300 mm
steel
F6700132
PROTEIN KW400G-4A
SEC
Hydroxyl
Silica
(guard)
5 µm
-
4.6 x 10 mm
steel
F6989303
PROTEIN LW-803
SEC
Hydroxyl
Silica
≥ 12,000
3 µm
300 Å (max. 1,000 Å)
8.0 x 300 mm
steel
F6700133
PROTEIN LW-G 6B
SEC
Hydroxyl
Silica
(guard)
3 µm
-
6.0 x 50 mm
steel
F6989403
PROTEIN LW-403 4D
SEC
Hydroxyl
Silica
≥ 11,000
1.9 µm
300 Å (max. 1,000 Å)
4.6 x 150 mm
steel
F6700134
PROTEIN LS-G 4J
SEC
Hydroxyl
Silica
(guard)
1.9 µm
-
4.6 x 20 mm
steel

Product details

Product name
Maximum pressure
Usual flow rate
Maximum flow rate
Temperature range
pH range
Salt concentration
Shipping solvent
USP
MW range
Exclusion limit
PROTEIN KW-802.5
5.0 MPa (50 bar)
1.0 mL/min
1.5 mL/min
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
5,000 - 100,000
150,000
PROTEIN KW-803
5.0 MPa (50 bar)
1.0 mL/min
1.5 mL/min
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
10,000 - 700,000
(1,000,000)
PROTEIN KW-804
5.0 MPa (50 bar)
1.0 mL/min
1.5 mL/min
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
30,000 - (4,000,000)
(4,000,000)
PROTEIN KW-G 6B
-
-
-
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
-
-
PROTEIN KW402.5-4F
10 MPa (100 bar)
0.35 mL/min
0.5 mL/min
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
5,000 - 70,000
150,000
PROTEIN KW403-4F
10 MPa (100 bar)
0.35 mL/min
0.5 mL/min
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
10,000 - 500,000
600,000
PROTEIN KW404-4F
7.0 MPa (70 bar)
0.35 mL/min
0.5 mL/min
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
30,000 - (4,000,000)
(4,000,000)
PROTEIN KW405-4F
7.0 MPa (70 bar)
0.35 mL/min
0.5 mL/min
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
200,000 - (20,000,000)
(20,000,000)
PROTEIN KW400G-4A
-
-
-
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
-
-
PROTEIN LW-803
15 MPa (150 bar)
1.0 mL/min
1.5 mL/min
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
10,000 - 700,000
(1,000,000)
PROTEIN LW-G 6B
-
-
-
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
-
-
PROTEIN LW-403 4D
20 MPa (200 bar)
0.35 mL/min
0.5 mL/min
5 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
10,000 - 700,000
(1,000,000)
PROTEIN LS-G 4J
-
-
-
4 to 45°C
3.0 to 7.5
≤ 0.3 M
H2O
L20, L33, L59
-
-

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