PROTEIN KW/ PROTEIN LW columns

separation by size also for high molecular weight

The silica-based SEC (GFC) column for proteins and enzymes

Product information

PROTEIN KW-800 series
The packing material of the Shodex PROTEIN KW-800 series is based on silica particles whose surface is coated with a hydrophilic polymer. This column is for aqueous SEC (Gel filtration chromatography (GFC)). It enjoys a positive reputation thanks to its high recovery ratio of proteins and peptide. The PROTEIN KW-800 series comprises three kinds of columns, differing in pore and particle size.

Silica-based packed columns for aqueous SEC (GFC) analysis
Suitable for the analysis of proteins and enzymes
Fulfills USP-NF L20, L33, and L59 requirements

PROTEIN KW400 series
The Shodex KW400-4F series is the high-performance semi-micro column version of the KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. KW405-4F, the newest addition to the KW400 line-up, has a larger pore size than any columns in the existing KW-800 series, and can analyze proteins whose molecular weight is larger than 1,000,000. It enables the scientist to carry out the analysis of protein complexes.

Reduced packing material particle size enhances column performance
Three to four-fold higher sensitivity than KW-800 series
KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
Fulfills USP-NF L20, L33, and L59 requirements

PROTEIN LW-803 and LW-403 4D

More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.

Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand
High performance analysis of antibody drugs and various proteins
High lot-to-lot reproducibility
Fulfills USP-NF L20, L33, and L59 requirements

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F6989000	PROTEIN KW-802.5	SEC	Hydroxyl	Silica	≥ 21,000	5 µm	150 Å (max. 400 Å)	8.0 x 300 mm	steel
F6989103	PROTEIN KW-803	SEC	Hydroxyl	Silica	≥ 21,000	5 µm	300 Å (max. 1,000 Å)	8.0 x 300 mm	steel
F6989104	PROTEIN KW-804	SEC	Hydroxyl	Silica	≥ 16,000	7 µm	500 Å (max. 1,500 Å)	8.0 x 300 mm	steel
F6700131	PROTEIN KW-G 6B	SEC	Hydroxyl	Silica	(guard)	7 µm	-	6.0 x 50 mm	steel
F6989201	PROTEIN KW402.5-4F	SEC	Hydroxyl	Silica	≥ 35,000	3 µm	150 Å (max. 400 Å)	4.6 x 300 mm	steel
F6989202	PROTEIN KW403-4F	SEC	Hydroxyl	Silica	≥ 35,000	3 µm	250 Å (max. 800 Å)	4.6 x 300 mm	steel
F6989203	PROTEIN KW404-4F	SEC	Hydroxyl	Silica	≥ 25,000	5 µm	500 Å (max. 1,500 Å)	4.6 x 300 mm	steel
F6989204	PROTEIN KW405-4F	SEC	Hydroxyl	Silica	≥ 25,000	5 µm	1,000 Å (max. 2,000 Å)	4.6 x 300 mm	steel
F6700132	PROTEIN KW400G-4A	SEC	Hydroxyl	Silica	(guard)	5 µm	-	4.6 x 10 mm	steel
F6989303	PROTEIN LW-803	SEC	Hydroxyl	Silica	≥ 12,000	3 µm	300 Å (max. 1,000 Å)	8.0 x 300 mm	steel
F6700133	PROTEIN LW-G 6B	SEC	Hydroxyl	Silica	(guard)	3 µm	-	6.0 x 50 mm	steel
F6989403	PROTEIN LW-403 4D	SEC	Hydroxyl	Silica	≥ 11,000	1.9 µm	300 Å (max. 1,000 Å)	4.6 x 150 mm	steel
F6700134	PROTEIN LS-G 4J	SEC	Hydroxyl	Silica	(guard)	1.9 µm	-	4.6 x 20 mm	steel

Product details

Product name	Maximum pressure	Usual flow rate	Maximum flow rate	Temperature range	pH range	Salt concentration	Shipping solvent	USP	MW range	Exclusion limit
PROTEIN KW-802.5	5.0 MPa (50 bar)	1.0 mL/min	1.5 mL/min	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	5,000 - 100,000	150,000
PROTEIN KW-803	5.0 MPa (50 bar)	1.0 mL/min	1.5 mL/min	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	10,000 - 700,000	(1,000,000)
PROTEIN KW-804	5.0 MPa (50 bar)	1.0 mL/min	1.5 mL/min	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	30,000 - (4,000,000)	(4,000,000)
PROTEIN KW-G 6B	-	-	-	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	-	-
PROTEIN KW402.5-4F	10 MPa (100 bar)	0.35 mL/min	0.5 mL/min	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	5,000 - 70,000	150,000
PROTEIN KW403-4F	10 MPa (100 bar)	0.35 mL/min	0.5 mL/min	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	10,000 - 500,000	600,000
PROTEIN KW404-4F	7.0 MPa (70 bar)	0.35 mL/min	0.5 mL/min	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	30,000 - (4,000,000)	(4,000,000)
PROTEIN KW405-4F	7.0 MPa (70 bar)	0.35 mL/min	0.5 mL/min	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	200,000 - (20,000,000)	(20,000,000)
PROTEIN KW400G-4A	-	-	-	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	-	-
PROTEIN LW-803	15 MPa (150 bar)	1.0 mL/min	1.5 mL/min	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	10,000 - 700,000	(1,000,000)
PROTEIN LW-G 6B	-	-	-	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	-	-
PROTEIN LW-403 4D	20 MPa (200 bar)	0.35 mL/min	0.5 mL/min	5 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	10,000 - 700,000	(1,000,000)
PROTEIN LS-G 4J	-	-	-	4 to 45°C	3.0 to 7.5	≤ 0.3 M	H2O	L20, L33, L59	-	-

Products

Download documents

Technical Articles:

Shodex Technical Article 1_Analysis of Aggregates and Additives in Antibody Drugs (pdf)

Shodex Technical Article 11_HPLC Analysis of Antidiabetic Drugs (pdf)

English Manuals:

Operation Manual PROTEIN KW400-4F Series_20221202E (pdf)

Operation Manual PROTEIN KW-800 Series_20221202E (pdf)

Operation Manual PROTEIN LW-403 4D_20221202E (pdf)

Operation Manual PROTEIN LW-803_20221202E (pdf)

German Manuals:

Bedienungsanleitung PROTEIN KW400-4F Serie_20221202G (pdf)

Bedienungsanleitung PROTEIN KW-800 Serie_20221202G (pdf)

Bedienungsanleitung PROTEIN LW-403 4D_20221202G (pdf)

Bedienungsanleitung PROTEIN LW-803_20221202G (pdf)

Product instruction

Usable solvents are listed below.
(1) The standard eluent is a buffer with a salt. Buffers such as phosphate, acetate, and Tris-HCl can be used.
(2) Salts such as sodium chloride, sodium sulfate, potassium sulfate and ammonium sulfate can be used. Please keep total concentration of salts under 1 M. Their recommended concentration ranges are 0.1 - 0.3 M.
(3) Up to 100 % (v/v) acetonitrile, methanol, ethanol, and 2-propanol (IPA) are usable.
(4) Protein denaturants such as urea and 6-M guanidine hydrochloride, and surfactants such as SDS and Brij-35 can also be used. However, since protein denaturants and surfactants tend to remain on the column, solvent replacement after their use takes a longer time than replacement from general solvents. It is recommended to dedicate the column for this specific use.

<Cleaning Method>
(1) Insoluble components that block the column inlet may be removed by reversing the flow direction, i.e., introducing the eluent from the column outlet, with flow rate at less than half of the recommended flow rate.
(2) Follow below cleaning steps for adsorbing components. For an efficient cleaning, reverse the flow direction and use the flow rate at less than half of the recommended flow rate. Introduce 5 to 10 times column volume of the washing solvent.
Method 1: Adsorption of ionic (basic) compounds
· Introduce the eluent with a salt concentration of 0.5 M.
· Introduce an acidic aqueous solution of pH 3.
Method 2: Adsorption of hydrophobic compounds
· Introduce the eluent with 10 - 20 % (v/v) organic solvent (acetonitrile or methanol).

Applications

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General PROTEIN KW-800

Effects of Flow Rate (KW-802.5, KW-803)

Recovery of Proteins (KW-802.5 and KW-803)

Recovery of Proteins (KW-803)

Sample Load (KW-803)

Standard Proteins (1) (KW-802.5)

Standard Proteins (14) (KW-802.5)

Standard Proteins (15) (KW-803)

Standard Proteins (16) (KW-804)

Standard Proteins (18) (KW-803)

Standard Proteins (19) (KW-804)

Standard Proteins (2) (KW-802.5 and KW-803)