PROTEIN KW/ PROTEIN LW columns
separation by size also for high molecular weight
The silica-based SEC (GFC) column for proteins and enzymes
Download the product overview (pdf)
Product information
PROTEIN KW-800 series
The packing material of the Shodex PROTEIN KW-800 series is based on silica particles whose surface is coated with a hydrophilic polymer. This column is for aqueous SEC (Gel filtration chromatography (GFC)). It enjoys a positive reputation thanks to its high recovery ratio of proteins and peptide. The PROTEIN KW-800 series comprises three kinds of columns, differing in pore and particle size.
-
Silica-based packed columns for aqueous SEC (GFC) analysis
-
Suitable for the analysis of proteins and enzymes
-
Fulfills USP-NF L20, L33, and L59 requirements
PROTEIN KW400 series
The Shodex KW400-4F series is the high-performance semi-micro column version of the KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. KW405-4F, the newest addition to the KW400 line-up, has a larger pore size than any columns in the existing KW-800 series, and can analyze proteins whose molecular weight is larger than 1,000,000. It enables the scientist to carry out the analysis of protein complexes.
-
Reduced packing material particle size enhances column performance
-
Three to four-fold higher sensitivity than KW-800 series
-
KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
-
Fulfills USP-NF L20, L33, and L59 requirements
PROTEIN LW-803 and LW-403 4D
More and more monoclonal antibodies (mAb) are used for therapeutic drugs. The pore size of the Shodex PROTEIN LW-803 and LW-403 4D is suitable for this analysis.
-
Pore size specifically controlled for analyzing proteins with a molecular weight of several hundred of thousand
-
High performance analysis of antibody drugs and various proteins
-
High lot-to-lot reproducibility
-
Fulfills USP-NF L20, L33, and L59 requirements
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F6989000 | PROTEIN KW-802.5 | SEC | Hydroxyl | Silica | ≥ 21,000 | 5 µm | 150 Å (max. 400 Å) | 8.0 x 300 mm | steel |
F6989103 | PROTEIN KW-803 | SEC | Hydroxyl | Silica | ≥ 21,000 | 5 µm | 300 Å (max. 1,000 Å) | 8.0 x 300 mm | steel |
F6989104 | PROTEIN KW-804 | SEC | Hydroxyl | Silica | ≥ 16,000 | 7 µm | 500 Å (max. 1,500 Å) | 8.0 x 300 mm | steel |
F6700131 | PROTEIN KW-G 6B | SEC | Hydroxyl | Silica | (guard) | 7 µm | - | 6.0 x 50 mm | steel |
F6989201 | PROTEIN KW402.5-4F | SEC | Hydroxyl | Silica | ≥ 35,000 | 3 µm | 150 Å (max. 400 Å) | 4.6 x 300 mm | steel |
F6989202 | PROTEIN KW403-4F | SEC | Hydroxyl | Silica | ≥ 35,000 | 3 µm | 250 Å (max. 800 Å) | 4.6 x 300 mm | steel |
F6989203 | PROTEIN KW404-4F | SEC | Hydroxyl | Silica | ≥ 25,000 | 5 µm | 500 Å (max. 1,500 Å) | 4.6 x 300 mm | steel |
F6989204 | PROTEIN KW405-4F | SEC | Hydroxyl | Silica | ≥ 25,000 | 5 µm | 1,000 Å (max. 2,000 Å) | 4.6 x 300 mm | steel |
F6700132 | PROTEIN KW400G-4A | SEC | Hydroxyl | Silica | (guard) | 5 µm | - | 4.6 x 10 mm | steel |
F6989303 | PROTEIN LW-803 | SEC | Hydroxyl | Silica | ≥ 12,000 | 3 µm | 300 Å (max. 1,000 Å) | 8.0 x 300 mm | steel |
F6700133 | PROTEIN LW-G 6B | SEC | Hydroxyl | Silica | (guard) | 3 µm | - | 6.0 x 50 mm | steel |
F6989403 | PROTEIN LW-403 4D | SEC | Hydroxyl | Silica | ≥ 11,000 | 1.9 µm | 300 Å (max. 1,000 Å) | 4.6 x 150 mm | steel |
F6700134 | PROTEIN LS-G 4J | SEC | Hydroxyl | Silica | (guard) | 1.9 µm | - | 4.6 x 20 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Salt concentration | Shipping solvent | USP | MW range | Exclusion limit |
|---|---|---|---|---|---|---|---|---|---|---|
PROTEIN KW-802.5 | 5.0 MPa (50 bar) | 1.0 mL/min | 1.5 mL/min | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 5,000 - 100,000 | 150,000 |
PROTEIN KW-803 | 5.0 MPa (50 bar) | 1.0 mL/min | 1.5 mL/min | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 10,000 - 700,000 | (1,000,000) |
PROTEIN KW-804 | 5.0 MPa (50 bar) | 1.0 mL/min | 1.5 mL/min | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 30,000 - (4,000,000) | (4,000,000) |
PROTEIN KW-G 6B | - | - | - | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | - | - |
PROTEIN KW402.5-4F | 10 MPa (100 bar) | 0.35 mL/min | 0.5 mL/min | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 5,000 - 70,000 | 150,000 |
PROTEIN KW403-4F | 10 MPa (100 bar) | 0.35 mL/min | 0.5 mL/min | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 10,000 - 500,000 | 600,000 |
PROTEIN KW404-4F | 7.0 MPa (70 bar) | 0.35 mL/min | 0.5 mL/min | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 30,000 - (4,000,000) | (4,000,000) |
PROTEIN KW405-4F | 7.0 MPa (70 bar) | 0.35 mL/min | 0.5 mL/min | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 200,000 - (20,000,000) | (20,000,000) |
PROTEIN KW400G-4A | - | - | - | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | - | - |
PROTEIN LW-803 | 15 MPa (150 bar) | 1.0 mL/min | 1.5 mL/min | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 10,000 - 700,000 | (1,000,000) |
PROTEIN LW-G 6B | - | - | - | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | - | - |
PROTEIN LW-403 4D | 20 MPa (200 bar) | 0.35 mL/min | 0.5 mL/min | 5 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | 10,000 - 700,000 | (1,000,000) |
PROTEIN LS-G 4J | - | - | - | 4 to 45°C | 3.0 to 7.5 | ≤ 0.3 M | H2O | L20, L33, L59 | - | - |