HILICpak VG-50 columns
with tertiary amino groups
The robust HILIC column for many general applications
Product information
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Suitable for saccharide analysis using HILIC mode
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Recovers reducing saccharides with high ratio (usage of tert. amine groups to avoid building of Schiff base)
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Polymer-based packing material provides excellent chemical stability and minimum deterioration
over an extended time period
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pH stability: 2 to 13 (silanol free)
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Easily regenerated by washing in an alkaline solution
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Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)
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With smaller packing material, VG-25 has higher separation capabilities
Product overview
Product code | Product name | Separation | Functional group | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|---|
F6711900 | HILICpak VG-25G 2A | HILIC | Amino | Polyvinyl alcohol | (guard) | 2.5 µm | - | 2.0 x 10 mm | PEEK |
F7631100 | HILICpak VG-25 2B | HILIC | Amino | Polyvinyl alcohol | ≥ 3,500 | 2.5 µm | 100 Å | 2.0 x 150 mm | PEEK |
F6711800 | HILICpak VG-25G 4A | HILIC | Amino | Polyvinyl alcohol | (guard) | 2.5 µm | - | 4.6 x 10 mm | PEEK |
F7631000 | HILICpak VG-25 4D | HILIC | Amino | Polyvinyl alcohol | ≥ 16,500 | 2.5 µm | 100 Å | 4.6 x 150 mm | PEEK |
F7630200 | HILICpak VG-50 4D | HILIC | Amino | Polyvinyl alcohol | ≥ 5,500 | 5 µm | 100 Å | 4.6 x 150 mm | steel |
F7630100 | HILICpak VG-50 4E | HILIC | Amino | Polyvinyl alcohol | ≥ 7,500 | 5 µm | 100 Å | 4.6 x 250 mm | steel |
F6711100 | HILICpak VG-50G 4A | HILIC | Amino | Polyvinyl alcohol | (guard) | 5 µm | - | 4.6 x 10 mm | steel |
F7630300 | HILICpak VG-50 2D | HILIC | Amino | Polyvinyl alcohol | ≥ 3,500 | 5 µm | 100 Å | 2.0 x 150 mm | PEEK |
F6711200 | HILICpak VG-50G 2A | HILIC | Amino | Polyvinyl alcohol | (guard) | 5 µm | - | 2.0 x 10 mm | PEEK |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Shipping solvent | USP |
|---|---|---|---|---|---|---|---|
HILICpak VG-25G 2A | - | 0.1 to 0.2 mL/min | 0.40 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 15/85 | - |
HILICpak VG-25 2B | 10 MPa (100 bar) | 0.1 to 0.2 mL/min | 0.40 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 15/85 | - |
HILICpak VG-25G 4A | - | 0.5 to 1.0 mL/min | 1.0 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 20/80 | - |
HILICpak VG-25 4D | 15 MPa (150 bar) | 0.5 to 1.0 mL/min | 1.0 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 20/80 | - |
HILICpak VG-50 4D | 10 MPa (100 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 20/80 | - |
HILICpak VG-50 4E | 15 MPa (150 bar) | 0.5 to 1.0 mL/min | 1.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 20/80 | - |
HILICpak VG-50G 4A | - | 0.5 to 1.0 mL/min | 1.5 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 20/80 | - |
HILICpak VG-50 2D | 15 MPa (150 bar) | 0.1 to 0.2 mL/min | 0.45 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 15/85 | - |
HILICpak VG-50G 2A | - | 0.1 to 0.2 mL/min | 0.45 mL/min | 4 to 60°C | 2 to 13 | H2O/CH3CN = 15/85 | - |
Products
Download documents
Shodex Technical Article 10_Analysis of Functional Sugars in Foods Using HILIC Mode (pdf)
Shodex Technical Article 12_Simultaneous LC-MS Analyses of Phosphorylated Saccharides (pdf)
Applications
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General
Features of HILICpak VG-50 Series (1) (Recovery of Reducing Sugar)
Features of HILICpak VG-50 Series (2) (Stability of Baseline using Corona Charged Aerosol Detector)
Effects of Eluent pH on HILIC Mode (VG-50 2D)
Elution Volume of Saccharides (VG-50 4E)
Features of VG-25 (Separation Comparison)
Elution Volume of Saccharides (VG-25 4D)
Sugars
Analysis of 2-Deoxy-D-glucose (VG-50 4E)
Analysis of Aldoses (VG-50 4E)
Analysis of Allantoin in a Commercial Disinfectant (VG-50 4E)
Analysis of Erythrose and Erythritol (VG-50 4E)
Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
Analysis of Maltodextrin (2) (VG-25 4D)
Analysis of Rare Sugar (2) (VG-50 4E)
Analysis of Trisaccharides (2) (VG-50 4E)
Galactooligosaccharides (1) (VG-50 4E)
Isomaltooligosaccharides (2) (VG-50 4E)
Maltooligosaccharides (2) (VG-50 4E)
Melamine and Related Substances (VG-50 4D)
Monosaccharides and Disaccharides (5) (VG-50 4E)
Quantification of Purified Stevia Extract (VG-50 4E)
Quantitative Analysis of Sucrose in Saccharated Iron Oxide Injection (VG-50 4E)
Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
Simultaneous Analysis of Lactose, Epilactose, and Lactulose (2) (VG-25 4D)
LC/MS
LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
LC/MS Analysis of Inositol Phosphates (VG-50 2D)
LC/MS/MS Analysis of Saccharides (VG-50 2D)
Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
TESTIMONIALS
Great unique sugar column
Review date: 31 Oct 2017 | HILICpak VG-50 4E
Application Area: Analyze sugars in food products
"One of our most used columns in our laboratory for the last year. Good separation, long life and reproducible results. This unique column separate all the sugars needed for our diary product analysis, even the glucose/galactose pair which is usually very hard to separate. At last, after using a lot of "sugar" columns, we found the one! This column is also fits well our CAD detector which needs a non bleeding column. Although not so cheap, it has a great value for money."
Ronny Dayag / Tnuva on www.selectscience.net