ODP2 columns

with small pores

The robust polymer-based RP column for small molecules in protein matrix

Product information

ODP2 HP columns

The Shodex ODP2 HP series offers polymer–based columns for reversed phase chromatography. The efficiency of ODP2 HP columns is improved over most resin-based columns and is comparable with that of silica-based ODS columns. They don't contain C18 functional groups, the separation comes from the particle itself.

ODP2 HP has a better retention of highly polar substances compared to most general purpose silica-based C18 columns. Silica-based C18 ODS columns are susceptible to protein adsorption, resulting in degradation of the column. ODP2 HP is designed to exclude protein and thus, ODP2 HP can be used for analysis of drugs in biological samples containing protein without rapid column deterioration. As ODP2 HP can be used with low salt concentration without loss of peak shapes, it is excellent for LC/MS analysis.

Provides a large theoretical plate number nearly twice as much as generally available polymer-based
reversed phase columns do
Offers enhanced retention of high polar substances compared to ODS columns
Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix
Ideal for LC/MS analysis of high polar compounds
Fulfills USP L39 requirements

The material is completely silanol-free and therefore proteins are not adsorbed in the column.

Product overview

Product code	Product name	Separation	Functional group	Gel material	Plates per column	Particle size	Pore size	Size (ID x length)	Housing material
F7622004	ODP2 HP-2B	RP	-	Polyhydroxy-methacrylate	≥ 3,000	5 µm	40 Å	2.0 x 50 mm	steel
F7622005	ODP2 HP-2D	RP	-	Polyhydroxy-methacrylate	≥ 7,000	5 µm	40 Å	2.0 x 150 mm	steel
F6714011	ODP2 HPG-2A	RP	-	Polyhydroxy-methacrylate	(guard)	5 µm	-	2.0 x 50 mm	steel
F7622001	ODP2 HP-4B	RP	-	Polyhydroxy-methacrylate	≥ 3,500	5 µm	40 Å	4.6 x 50 mm	steel
F7622002	ODP2 HP-4D	RP	-	Polyhydroxy-methacrylate	≥ 10,000	5 µm	40 Å	4.6 x 150 mm	steel
F7622003	ODP2 HP-4E	RP	-	Polyhydroxy-methacrylate	≥ 17,000	5 µm	40 Å	4.6 x 250 mm	steel
F6714010	ODP2 HPG-4A	RP	-	Polyhydroxy-methacrylate	(guard)	5 µm	-	2.0 x 50 mm	steel

Product details

Product name	Maximum pressure	Maximum flow rate	Temperature range	pH range	Salt concentration	Shipping solvent	USP
ODP2 HP-2B	12 MPa (120 bar)	0.3 mL/min	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HP-2D	15 MPa (150 bar)	0.3 mL/min	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HPG-2A	-	-	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HP-4B	15 MPa (150 bar)	1.5 mL/min	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HP-4D	15 MPa (150 bar)	1.5 mL/min	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HP-4E	15 MPa (150 bar)	1.0 mL/min	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39
ODP2 HPG-4A	-	-	20 to 60°C	2 to 12	≤ 0.1 M	H2O/ACN=55/45	L39

Product info

Product instruction

Eluents:

ODP2 HP may be used with water, acid, base and aqueous salt solutions including most popular buffers, acetonitrile, methanol and mixtures of these components.

<Representative Acids> Phosphoric Acid, Formic Acid, Acetic Acid, and Trifluoroacetic Acid
<Representative Bases> Ammonia
<Representative Buffers> Phosphate Buffer, Formate Buffer, Acetate Buffer, and Carbonate Buffer
<Polar Organic Solutions> Methanol, Acetonitrile

Precautions
1) Eluent should be in the pH range of 3~12.
2) The total concentration of acid, base, and salt should be 100 mM or less. Generally, a range of 1~50 mM is recommended.
3) When adding acetonitrile or methanol to the aqueous salt solution, confirm there is no salt precipitation before use.
4) Nonpolar organic substances such as hexane or toluene cannot be used.

Download documents

Shodex Technical Notebook No.6 (ODP2, high performance RP for LC-MS) (pdf)

Operation Manual ODP2 HP-2B, 2D_20221202E

Operation Manual ODP2 HP-4B, 4D, 4E_20221202E

Operation Manual ODP2 HP-10E_prep_20221202E

Bedienungsanleitung ODP2 HP-2B, 2D_20221202G

Bedienungsanleitung ODP2 HP-4B, 4D, 4E_20221202G

Bedienungsanleitung ODP2 HP-10E_prep_20221202G

Applications

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