GPC LF linear columns
with multi-pore gel
The GPC column with highly linear calibration curve
Product information
GPC LF-404, GPC LF-604, GPC LF-804
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Packed with unique multi-pore gels with a wide pore-size distribution
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Highly linear calibration curve without inflection points
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Achieves highly precise molecular weight distribution determination
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Enables analysis over a broad range of molecular weights
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Rapid analysis column (LF-604) and high performance analysis column (LF-404) are also available
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LF-604 and LF-404 enables reduction of solvent use
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Fulfills USP L21 requirements
2 to 4 columns can be connected in series according to separation purpose or samples.
Analytes: all polymers, which are soluble in one of the mentioned organic solvents
This column series has similar properties like the following references: Tosoh TSKgel Multipore Hxl-M
Product overview
Product code | Product name | Separation | Gel material | Plates per column | Particle size | Pore size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|---|
F6021043 | GPC LF-404 | GPC | Styrene divinylbenzene copolymer | ≥ 14,000 | 6 µm | 3,000 Å | 4.0 x 250 mm | steel |
F6021042 | GPC LF-604 | GPC | Styrene divinylbenzene copolymer | ≥ 9,000 | 6 µm | 3,000 Å | 6.0 x 150 mm | steel |
F6021041 | GPC LF-804 | GPC | Styrene divinylbenzene copolymer | ≥ 17,000 | 6 µm | 3,000 Å | 8.0 x 300 mm | steel |
F6709621 | GPC LF-G | GPC | Styrene divinylbenzene copolymer | (guard) | 6 µm | - | 4.6 x 10 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | Shipping solvent | USP | MW range | Exclusion limit |
|---|---|---|---|---|---|---|---|---|
GPC LF-404 | 3.5 MPa (35 bar) | 0.3 mL/min | 0.6 mL/min | 20 to 60°C | THF | L21 | 300 - 2,000,000 | 2,000,000 |
GPC LF-604 | 3.5 MPa (35 bar) | 0.5 mL/min | 1.0 mL/min | 20 to 60°C | THF | L21 | 300 - 2,000,000 | 2,000,000 |
GPC LF-804 | 3.5 MPa (35 bar) | 1.0 mL/min | 2.0 mL/min | 20 to 60°C | THF | L21 | 300 - 2,000,000 | 2,000,000 |
GPC LF-G | - | - | - | 20 to 60°C | THF | L21 | - | - |
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Product instruction
Eluents:
The shipping solvent is Tetrahydrofuran (THF). The in-column solvent can be replaced. Following eluents are suitable:
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THF
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Chloroform
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Carbon tetrachloride
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Toluene
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Methyl ethyl ketone
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Acetone
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Dimethylformamide (DMF) Usable at 40°C or higher)
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Dimethylacetamide (DMAc) (Usable at 40°C or higher)
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Hexafluoroisopropanol (HFIP) (Usable at 40°C or higher)
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N-Methylpyrrolidone (NMP) (Usable at 40°C or higher)
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Dimethylsulfoxide (DMSO) (Usable at 40°C or higher)
Guard column:
The use of the guard column GPC LF-G is recommended.
Cleaning and Regeneration:
Clean the guard column and analytical column separately by running the eluent at 50% of standard flow rate in the opposite direction of the arrow on the column tag in order to remove the clogged substance.
Applications
Calibration Curve for LF-804 (DMAc Eluent: PEG, PEO)
Calibration Curve for LF-804 (DMAc Eluent: PS)
Calibration Curve for LF-804 (DMF Eluent: PEG, PEO)
Calibration Curve for LF-804 (DMSO/DMF Eluent: Pullulan)
Calibration Curve for LF-804 (Methyl Ethyl Ketone Eluent: PMMA)
Calibration Curve for LF-804 (NMP Eluent: PEG, PEO)
Calibration Curve for LF-804 (NMP Eluent: PS)
Calibration Curve for LF-804 (THF Eluent: PS)
Calibration Curve for LF-404 (HFIP Eluent: PMMA)
Calibration Curves for LF-404 and LF-604 (THF Eluent: PS)
Comparison of LF-804, LF-404 and LF-604
GPC LF-804 (8.0 x 300 mm)
Analysis of Polyhydroxybutyric Acid (LF-804)
Poly(2-Vinylpyridine-co-styrene) (LF-804)
Poly(4-Vinylpyridine) (LF-804)
Poly(Ethylene Oxid) and Poly(Ethylene Glycol) Standards (2) (LF-804)
Poly(Methyl Methacrylate) (2) (Eluent: Methyl Ethyl Ketone)
Poly(Vinyl Butyral) (2) (LF-804)
Poly(Vinyl Formal) (2) (LF-804)
Polycarbonate Resin (3) (LF-804)
Polystyrene Standards (22) (DMAc Eluent)
Pullulan Standards (8) (LF-804)
Separation of Polystyrene Standard using GPC Columns
GPC LF-604 (6.0 x 150 mm)
Biodegradable Polymer (2) (Polylactic Acid) (LF-604)
Comparison of Analysis of PMMA with Down-sized SEC Columns
Poly(Vinyl Butyral) (3) (LF-604)
Polyamide (5) Nylon 6/6 (LF-604)
Polyamide (6) Nylon 6 (LF-604)
GPC LF-404 (4.6 x 250 mm)
1,2-Polybutadiene (2) (LF-404)
Effects of The Unit of Columns on The Separation (1) (LF-404: PS)
Effects of The Unit of Columns on The Separation (2) (LF-404: Epoxy Resin)
Ethylene Vinyl Acetate Copolymer (3) (LF-404)
Poly(Ethylene Terephthalate) (3) (LF-404)
Calibration Curve for LF-804
(THF Eluent: PS)
Calibration curves for GPC LF-804 using polystyrene is shown. The good linear line can be obtained in the range of molecular weights 300-2,000,000.
Sample: Polystyrene, PS
Columns: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: THF
Flow rate: 1.0 mL/min
Detector: Shodex RI
Column temp.: 40°C
Calibration Curve for LF-804
(DMAc Eluent: PS)
The calibration curve for GPC LF-804, an organic SEC (GPC) column, using polystyrene is shown. The eluent is N,N-dimethylacetamide (DMAc).
Sample: Polystyrene, PS
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: 30 mM LiBr in DMAc
Flow rate: 0.7 mL/min
Detector: Shodex RI
Column temp.: 40°C
Calibration Curve for LF-804
(NMP Eluent: PS)
Calibration curve for GPC LF-804 using polystyrene is shown.
Sample: Polystyrene
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: NMP
Flow rate: 0.7 mL/min
Detector: Shodex RI
Column temp.: 50°C
Calibration Curve for LF-804
(DMSO/DMF Eluent: Pullulan)
Calibration curves for GPC LF-804, an organic SEC (GPC) column, using pullulan is shown. When starch is analyzed, DMSO/DMF mixture is used often for the eluent. LF-804 can be usable in DMSO/DMF mixture.
Sample: Pullulan, Maltose, Glucose
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: 10 mM LiBr in DMSO/DMF=75/25
Flow rate: 0.6mL/min
Detector: Shodex RI
Column temp.: 50°C
Calibration Curve for LF-804
(DMF Eluent: PEG, PEO)
Calibration curve for GPC LF-804 using PEG/PEO is shown.
Sample: Poly(ethylene oxide), Poly(ethylene glycol), Tetraethylene glycol
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: DMF
Flow rate: 0.7 mL/min
Detector: Shodex RI
Column temp.: 50°C
Calibration Curve for LF-804
(DMAc Eluent: PEG, PEO)
The calibration curve for GPC LF-804, an organic SEC (GPC) column, using PEG/PEO is shown. The eluent is N,N-dimethylacetamide (DMAc).
Sample: Poly(ethylene oxide), Poly(ethylene glycol), Tetraethylene glycol
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: 30 mM LiBr in DMAc
Flow rate: 0.7 mL/min
Detector: Shodex RI
Column temp.: 40°C
Calibration Curve for LF-804
(NMP Eluent: PEG, PEO)
Calibration curve for GPC LF-804 using PEG/PEO is shown.
Sample: Poly(ethylene oxide), Poly(ethylene glycol)
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: NMP
Flow rate: 0.7 mL/min
Detector: Shodex RI
Column temp.: 50°C
Calibration Curve for LF-804
(Methyl Ethyl Ketone Eluent: PMMA)
The calibration curve for GPC LF-804, an organic SEC (GPC) column, using poly(methyl methacrylate) is shown. The eluent is methyl ethyl ketone.
Sample: Poly(methyl methacrylate)
Column: Shodex GPC LF-804
(8.0 x 300 mm)
Eluent: Methyl ethyl ketone
Flow rate: 1.0mL/min
Detector: Shodex RI
Column temp.: 40°C
Calibration Curves for LF-404 and LF-604
(THF Eluent: PS)
GPC LF-404 and LF-604 are downsized columns of LF-804. Calibration curves for GPC LF-404 and LF-604 using polystyrene are shown. They show good linear lines as same as LF-804.
Sample: Polystyrene, PS
Columns: Shodex GPC LF-604 (6.0 x 150 mm),
LF-404 (4.6 x 250 mm)
Eluent: THF
Flow rate: 0.5mL/min (LF-604), 0.3mL/min (LF-404)
Detector: Shodex RI (Small cell volume)
Column temp.: 40°C
Calibration Curve for LF-404
(HFIP Eluent: PMMA)
Calibration curve for GPC LF-404 using Poly(methyl methacrylate) is shown.
Sample: Poly(methyl methacrylate)
Column: Shodex GPC LF-404 (4.6 x 250mm)
Eluent: HFIP
Flow rate: 0.3mL/min
Detector: Shodex RI
Column temp.: 40°C
Alternatives
Other single-pore columns with a smaller MW range are available from the GPC KF-800 series (THF), the GPC KD-800 series (DMF) or the ultra-rapid GPC HK-400 series.
Related products
The Shodex STANDARD S-Series is a calibration standard kit for organic size exclusion chromatography.
The Polystyrene (n = 4 to 200,000) is easily soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzene (ODCB).
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For organic solvent SEC (GPC)
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Less branched polystyrene with anionic polymerization
STANDARD SL-105 Kit: 10 vials with 0.5 g each (for low MW)
MW 23.000, 13.000, 10.000, 6.500, 4.900, 2.900, 2.000, 1.200, 1.100, 600
STANDARD SM-105 Kit: 10 vials with 0.5 g each (for medium MW)
MW 2.330.000, 1.700.000, 740.000, 321.000, 129.000, 57.000, 23.000, 6.500, 2.900, 1.200
STANDARD SH-75 Kit: 7 vials with 0.5 g each (for high MW)
MW 6.550.000, 3.550.000, 3.020.000, 2.330.000, 1.860.000, 885.000, 662.000
The Shodex STANDARD M-75 is Polymethylmethacrylate for SEC, and it is suitable for SEC using hexafluoroisopropanol (HFIP) and dimethylformamide (DMF) solvent. Other usable solvents are tetrahydrofuran (THF), toluene, methyl ethyl ketone, ethyl acetate, DMF, and DMAc.
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For organic solvent SEC (GPC)
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Narrow molecular weight distribution range
Note: Molecular weights (Mp, Mw/Mn) of a standard kit or each single type may vary depending on production lots. Always refer to the inspection certificate.
STANDARD M-75 Kit: 7 vials with 0.5 g each.
