Symbolic image of polar compound analysis

HILIC (hydrophilic interaction liquid chromatography)

Polymer-based columns for the analysis of polar substances

in drugs, food or biological samples

1. Product overview

2. Tutorial video

3. Theory of HILIC

1. Product overview

Shodex Asahipak NH2P Series.jpg

Asahipak NH2P-50 series

Polymer-based HILIC column with amino groups (-NH2)

  • For polar substances

  • Suitable for saccharides analysis using HILIC mode

  • Polymer-based packing material provides excellent chemical stability and minimum
    deterioration over extended time period

  • Easily regenerated by washing in an alkaline solution

  • Appropriate for ELSD, CAD, LC-MS/MS

  • Fulfills USP L82 requirement

 

HILICpak VG-50

Polymer-based HILIC columns with tertiary amino (-NR2) groups

  • Suitable for saccharide analysis using HILIC mode

  • Recovers reducing saccharides with high ratio (improved results for galactose, mannose, arabinose and xylose in comparison to Asahipak NH2P-50 series)

  • Best for melamine analysis

  • Appropriate for ELSD, CAD, LC-MS/MS

  • Steel and PEEK housings to avoid interaction

Shodex HILICpak Series.jpg
Shodex HILICpak Series.jpg

HILICpak VT-50

Polymer-based HILIC columns with quaternary ammonium (-NR3+) groups

  • Best for glyphosate analysis and phosphorylated sugars

  • Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode

  • Use of some eluents add ion exchange mode

  • Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period

  • Suitable for LC-MS/MS analysis

HILICpak VC-50

Polymer-based HILIC columns with carboxyl (-COO-) groups

  • Modified carboxyl group is suitable for cationic substance analysis including amines

  • The dominant separation mode is RP or IEX rather than HILIC mode

  • Best for aminoglycoside antibiotics, histamine/histidine, monoamine neurotransmitters and more

  • Suitable for LC-MS/MS analysis

Shodex HILICpak Series.jpg
Shodex HILICpak Series.jpg

HILICpak VN-50

Polymer-based HILIC columns with diol (-OH) groups

  • Best for oligosaccharides and oligonucleotides

  • Suitable for oligomer separation which is not possible by SEC column or conventional HILIC columns

  • The modified diol groups on the packing material create the HILIC mode

2. Tutorial video

 

3. Theory about HILIC

HILIC is a relatively new concept as a member of partition chromatography. It is considered as a part of normal phase because of its high polarity on the gel surface. The base material can be either silica or polymer and they may be modified with different types of polar functionalities such as amide, amino, diol, and cyano.

 

Compared to normal mode, the mobile phase used for HILIC is very similar to RP mode mobile phase such as mixture of water and acetonitrile. From practical view point, HILIC sits somehow between the RP and normal mode separation. Hydrophilic compounds that were “too polar” to be retained by RP can be analyzed by HILIC using the mobile phase similar to RP condition. Because of this feature HILIC is popularly used for the separation of carbohydrates, especially saccharide which is hydrophilic.


Shodex carries a polymer based amino column, named Asahipak NH2P series. NH2 indicates "amino functional group" and P again stands for polymer. This column is filled with a polyvinyl alcohol based gel, modified with polyamine. As for RP columns, polymer base compared to silica-base column will provide longer column life, durability in alkaline condition, and good repeatability.