Methylxanthine (1)

Methylxantines were analyzed using RSpak DS-613 (a column for reversed phase chromatography).


Sample :
1. Xanthine
2. Theobromine
3. Theophylline
4. Caffeine

Column       : Shodex RSpak DS-613 (6.0mmID*150mm)
Eluent       : H2O/CH3CN=88/12
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 40°C