UB Series
separation by size for biomolecules above and below 50 nm
Polymer-based Aqueous SEC (GFC) Columns for Bionanoproduct Analysis and Preparative Use
Product information
UB Series
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Suitable pore design for separating bio-nanoproducts such as viruses, virus-like particles (VLPs), and exosomes
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The uniform particles achieved both low back pressure and high separation efficiency
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High stability against basic solvents and other chemicals commonly used in bioprocessing
Eluent conditions
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Buffers and aqueous solutions of different salts can be used separately or together. Usable buffers include phosphate, acetate, citrate, and Tris. Usable aqueous salt solutions include sodium chloride, sodium sulfate, potassium sulfate, and ammonium sulfate. Their recommended concentration ranges are 0.05 to 0.3 M
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Up to 50 % (v/v) methanol, ethanol, isopropanol and acetonitrile are usable.
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Aqueous solutions of urea, guanidine hydrochloride, and dithiothreitol (DTT) which are the protein denaturants can be used up to 8 M, 6 M, and 5 mM, respectively. However, their required concentration are usually high, which may damage the column during the solvent replacement. Thus, it is recommended to dedicate the column for these specific uses.
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SDS surfactant can also be added up to 2% (w/v). However, since surfactants tend to remain on the column, solvent replacement after their use takes a longer time than replacement from general solvents. The replacement time can be shorten by using 30 to 50 % (v/v) methanol.
Flyer new UB columns: Shodex UB flyer_e.pdf
Product overview
Product code | Product name | Separation | Gel material | Plates per column | Particle size | Size (ID x length) | Housing material |
|---|---|---|---|---|---|---|---|
F6429301 | UB-50 | SEC | Polyhydroxymethacrylate | ≥ 3,500 | 26 µm | 8 x 300 mm | steel |
F6429302 | UB-100 | SEC | Polyhydroxymethacrylate | ≥ 3,500 | 27 µm | 8 x 300 mm | steel |
Product details
Product name | Maximum pressure | Usual flow rate | Maximum flow rate | Temperature range | pH range | Shipping solvent | Target Molecular Size |
|---|---|---|---|---|---|---|---|
UB-50 | 0.5 MPa (5 bar) | 0.5 to 1.0 mL/min | 2.0 mL/min | 4 to 60°C | 2 to 14 | H2O | < 50 nm |
UB-100 | 0.5 MPa (5 bar) | 0.5 to 1.0 mL/min | 2.0 mL/min | 4 to 60°C | 2 to 14 | H2O | > 50 nm |
Download documents
Operation Manuals:
Operation manuals for each column series can be found on our Japanese website.
Please click the following link to be directed to the Operation Manual Database.
Applications
Please take a look at the following list of example application data and chromatograms obtained with our columns. These links will redirect you to our Japanese website