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Technical Specifications


PROTEIN KW400 series
High-performance semi-micro column version of the PROTEIN KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. 


  • Silica-based packed columns for aqueous SEC (GFC) analysis

  • Suitable for the analysis of proteins and enzymes

  • Reduced packing material particle size enhances column performance

  • Three to four-fold higher sensitivity than KW-800 series

  • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000

  • Fulfills USP L20, L33, and L59 requirements


Product code: F6989204

Product name: PROTEIN KW405-4F

Separation: SEC

Functional group: Hydroxyl

Gel material: Silica

Plates per column: ≥ 25,000

Particle size: 5 µm

Pore size: 1,000 Å (max. 2,000 Å)

Size (ID x length): 4.6 x 300 mm

Housing material: steel

Maximum pressure: 7.0 MPa (70 bar)

Usual flow rate: 0.35 mL/min

Maximum flow rate: 0.5 mL/min

Temperature range: 5 to 45°C

pH range: 3.0 to 7.5

Salt concentration: ≤ 0.3 M

Shipping solvent: H2O

USP: L20, L33, L59

MW range: 200,000 - (20,000,000)

Exclusion limit: (20,000,000)


This column can be coupled with another column of this series.


The corresponding guard column is PROTEIN KW400G-4A, F6700132.

PROTEIN KW405-4F | 4.6 x 300 mm

SKU: F6989204
Excluding VAT

Where to buy?


Shodex HPLC columns and calibration standards are available at many different laboratory equipment suppliers.

Find your distributor for countries in Europe, Middle East and Africa and contact them directly:

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